OBJECTIVE: The current study sought to investigate the role of estrogen in the formation of experimental abdominal aortic aneurysm (AAA). METHODS: Elastase perfusion of infrarenal AAA animal model was performed in 20 female and 20 male Wistar rats that were randomly divided into an ovariectomized/sham-operated group and an estradiol (E2) experimental/saline control group, respectively. At day 14, E2 was detected, while the mRNA and protein expressions of matrix metalloproteinases 2 and 9 (MMP-2 and -9) in AAA tissue were detected by immunohistochemistry and polymerase chain reaction (PCR). RESULTS: The ovariectomized group showed lower estrogen levels and a higher aneurysm dilatation rate and significantly higher MMP-2 and -9 expression compared with the sham-operated group (P < .01), which was in accordance with MMP-2 and -9 mRNA expression. The E2 group showed higher estrogen levels and a lower aneurysm dilatation rate and significantly lower MMP-2 and -9 expression than did the saline control group (P < .01), which was in accordance with MMP-2 and -9 mRNA expression. CONCLUSIONS: In the pathogenesis of AAA, estrogen may play an inhibitory role by decreasing expression of MMP-2 and MMP-9 synthesis.
OBJECTIVE: The current study sought to investigate the role of estrogen in the formation of experimental abdominal aortic aneurysm (AAA). METHODS: Elastase perfusion of infrarenal AAA animal model was performed in 20 female and 20 male Wistar rats that were randomly divided into an ovariectomized/sham-operated group and an estradiol (E2) experimental/saline control group, respectively. At day 14, E2 was detected, while the mRNA and protein expressions of matrix metalloproteinases 2 and 9 (MMP-2 and -9) in AAA tissue were detected by immunohistochemistry and polymerase chain reaction (PCR). RESULTS: The ovariectomized group showed lower estrogen levels and a higher aneurysm dilatation rate and significantly higher MMP-2 and -9 expression compared with the sham-operated group (P < .01), which was in accordance with MMP-2 and -9 mRNA expression. The E2 group showed higher estrogen levels and a lower aneurysm dilatation rate and significantly lower MMP-2 and -9 expression than did the saline control group (P < .01), which was in accordance with MMP-2 and -9 mRNA expression. CONCLUSIONS: In the pathogenesis of AAA, estrogen may play an inhibitory role by decreasing expression of MMP-2 and MMP-9 synthesis.
Authors: Abhijit Ghosh; Guanyi Lu; Gang Su; Brendan McEvoy; Omar Sadiq; Paul D DiMusto; Adriana Laser; John S Futchko; Peter K Henke; Jonathan L Eliason; Gilbert R Upchurch Journal: Am J Pathol Date: 2014-01 Impact factor: 4.307
Authors: Kristian A Groth; Kirstine Stochholm; Hanne Hove; Kasper Kyhl; Pernille A Gregersen; Niels Vejlstrup; John R Østergaard; Claus H Gravholt; Niels H Andersen Journal: Clin Res Cardiol Date: 2016-08-22 Impact factor: 5.460
Authors: William F Johnston; Morgan Salmon; Gang Su; Guanyi Lu; Gorav Ailawadi; Gilbert R Upchurch Journal: J Vasc Surg Date: 2014-02-28 Impact factor: 4.268