Microencapsulation of human fibroblasts in a water-insoluble polyacrylate.

Viable human diploid fibroblasts have been micro-encapsulated in EUDRAGIT RL, a commercially available water-insoluble polyacrylate, by an interfacial precipitation technique. Cells in medium and polymer solution (in diethyl phthalate) were coextruded and formed into droplets by a coaxial air stream. The droplets fell into a corn-oil/mineral-oil mixture to extract the solvent to precipitate the polymer around the cells. Capsules were ca. 500 mum in diameter depending on the air flowrate with a ca. 10-mum thick wall. When collagen (1 mg/mL) was added to the cell suspension prior to encapsulation and base-washed corn oil was used, cell growth occurred with one doubling achieved after five to six days as the collagen gel contracted inside the capsule. In the absence of collagen, cells spread on the inner wall of the capsule but did not grow, presumably because the surface charge on the capsule was inadequate. In similar fashion fibroblasts spread but did not grow on films of EUDRAGIT RL but did grow on blends of EUDRAGIT RL and EUDRAGIT E containing 10-30% of the latter more highly aminated polyacrylate. Although not suitable for anchorage-dependent cell growth by itself, EUDRAGIT RL has been suitable as a model polymer to demonstrate the feasibility of using water insoluble polyacrylates and organic solvents and nonsolvents for the micro-encapsulation of fibroblasts. Such microcapsules are of potential interest as a mode of large scale tissue culture for the production of novel therapeutic agents.