Continuous cellulase production by immobilized Sporotrichum cellulophilum and continuous saccharification of bagasse.

Immobilized Sporotrichum cellulophilum with nonwoven materials was cultured continuously by a rotating-disk fermentor to supply cellulase into the saccharification system. The filter paper activity (5.0) was retained after 696 h under conditions of 250 rpm stirring and 0.014 h(-1) dilution rate. The product of the culture was supplied continuously to the saccharification reactor and used for the saccharification of bagasse. A glucose solution of ca. 0.9% was obtained continuously from 5% bagasse slurry during 610 h saccharification by this method.