Literature DB >> 18576763

Molecular characterization of Toxoplasma gondii isolates from cats in Spain.

A Montoya1, G Miró, M Mateo, C Ramírez, I Fuentes.   

Abstract

Cats are important in the epidemiology of Toxoplasma gondii because felids are the only definitive hosts that can excrete environmentally resistant oocysts. Fresh samples of brain from 103 Spanish cats with antibodies to T. gondii were analyzed for T. gondii DNA using nested-PCR; 47 (45.5%) were found to be positive. Further characterization of DNA from 46 cats using RFLP-PCR at the 3' and 5' ends of the SAG2 locus revealed that 12 (26%) isolates were Type I and 34 (74%) were Type II; no Type III were found, and the 47th sample could not be classified to its genetic type. In addition, T. gondii was also isolated by bioassay in mice from 42 of 103 seropositive cats. This is the first report of T. gondii characterization from cats in Spain.

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Year:  2008        PMID： 18576763     DOI： 10.1645/GE-1403.1

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  5 in total

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Authors:  Hye-Youn Kim; Yun-Ah Kim; Ho Sa Lee; Ho Gun Rhie; Shin-Hyeong Cho; Jae-Ran Yu; Sang-Eun Lee
Journal:  Korean J Parasitol       Date:  2009-12-02       Impact factor: 1.341

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Journal:  Vet Res       Date:  2012-04-30       Impact factor: 3.683

3.  Genotyping Characterization of Toxoplasma gondii in Cattle, Sheep, Goats and Swine from the North of Portugal.

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Journal:  Iran J Parasitol       Date:  2015 Jul-Sep       Impact factor: 1.012

4.  Virulence in Mice of a Toxoplasma gondii Type II Isolate Does Not Correlate With the Outcome of Experimental Infection in Pregnant Sheep.

Authors:  Roberto Sánchez-Sánchez; Ignacio Ferre; Javier Regidor-Cerrillo; Daniel Gutiérrez-Expósito; Luis Miguel Ferrer; Noive Arteche-Villasol; Javier Moreno-Gonzalo; Joachim Müller; Adriana Aguado-Martínez; Valentín Pérez; Andrew Hemphill; Luis Miguel Ortega-Mora; Julio Benavides
Journal:  Front Cell Infect Microbiol       Date:  2019-01-04       Impact factor: 5.293

5.  Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks.

Authors:  Mercedes Fernández-Escobar; Rafael Calero-Bernal; Julio Benavides; Javier Regidor-Cerrillo; María Cristina Guerrero-Molina; Daniel Gutiérrez-Expósito; Esther Collantes-Fernández; Luis Miguel Ortega-Mora
Journal:  Parasit Vectors       Date:  2020-08-05       Impact factor: 3.876
  5 in total

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