[Isolation and identification of major steroidal saponins of Paris polyphylla Smith var stenophylla Franch].

OBJECTIVE: To research the steroidal saponins of Paris polyphylla Smith var stenophylla Franch for enriching the resource of Radix Paridis.
METHODS: Compounds were isolated by silica gel, macroporous adsorption resin,Sephadex LH-20 and RP-C18 column chromatography and the structures were elucidated on the basis of spectroscopic methods.
RESULTS: Five kinds of steroidal saponins were isolated and identified as follows: paris saponin I (1) Diosgenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[alpha-L-arabinofuranosyl-(1 --> 4 )]-beta-D-Glucopyranoside, paris saponin V (2) Diosgenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside, paris saponin VI (3) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside, paris saponin VII (4) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl- 1 --> 4)-[alpha-L-rh-amnopyranosyl-(1 --> 2)]-beta-D-glucopyranoside, paris saponin H (5) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[alpha-L-arabinofuranosyl-(1 --> 4)]-beta-D-Glucopyranoside.
CONCLUSION: All the five kinds of steroidal saponins were isolated from P. polyphylla. Smith var stenophylla Franch for the first time.