Literature DB >> 18574630

Flow cytometry as a rapid test for detection of penicillin resistance directly in bacterial cells in Enterococcus faecalis and Staphylococcus aureus.

T Jarzembowski1, K Wiśniewska, A Józwik, E Bryl, J Witkowski.   

Abstract

We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescence values per particle in penicillin-resistant than in penicillin-sensitive strains of both species observed. The method allows rapid detection of penicillin resistance in S. aureus and E. faecalis. The results encourage further investigations on the detection of antibiotic resistance in bacteria using flow cytometry.

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Year:  2008        PMID： 18574630     DOI： 10.1007/s00284-008-9179-8

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  11 in total

1.  BOCILLIN FL, a sensitive and commercially available reagent for detection of penicillin-binding proteins.

Authors:  G Zhao; T I Meier; S D Kahl; K R Gee; L C Blaszczak
Journal:  Antimicrob Agents Chemother       Date:  1999-05       Impact factor: 5.191

Review 2.  Beta-lactamase-producing enterococci.

Authors:  B E Murray
Journal:  Antimicrob Agents Chemother       Date:  1992-11       Impact factor: 5.191

3.  Modification of penicillin-binding proteins of penicillin-resistant mutants of different species of enterococci.

Authors:  S al-Obeid; L Gutmann; R Williamson
Journal:  J Antimicrob Chemother       Date:  1990-11       Impact factor: 5.790

4.  Evidence for clonal spread of a single strain of beta-lactamase-producing Enterococcus (Streptococcus) faecalis to six hospitals in five states.

Authors:  B E Murray; K V Singh; S M Markowitz; H A Lopardo; J E Patterson; M J Zervos; E Rubeglio; G M Eliopoulos; L B Rice; F W Goldstein
Journal:  J Infect Dis       Date:  1991-04       Impact factor: 5.226

5.  Origin and molecular epidemiology of penicillin-binding-protein-mediated resistance to beta-lactam antibiotics.

Authors:  C G Dowson; T J Coffey; B G Spratt
Journal:  Trends Microbiol       Date:  1994-10       Impact factor: 17.079

6.  Structure of the low-affinity penicillin-binding protein 5 PBP5fm in wild-type and highly penicillin-resistant strains of Enterococcus faecium.

Authors:  W Zorzi; X Y Zhou; O Dardenne; J Lamotte; D Raze; J Pierre; L Gutmann; J Coyette
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

7.  The penicillin resistance of Enterococcus faecalis JH2-2r results from an overproduction of the low-affinity penicillin-binding protein PBP4 and does not involve a psr-like gene.

Authors:  Colette Duez; Willy Zorzi; Frédéric Sapunaric; Ana Amoroso; Iris Thamm; Jacques Coyette
Journal:  Microbiology (Reading)       Date:  2001-09       Impact factor: 2.777

Review 8.  Applications of flow cytometry to clinical microbiology.

Authors:  A Alvarez-Barrientos; J Arroyo; R Cantón; C Nombela; M Sánchez-Pérez
Journal:  Clin Microbiol Rev       Date:  2000-04       Impact factor: 26.132

9.  Role of class A penicillin-binding proteins in PBP5-mediated beta-lactam resistance in Enterococcus faecalis.

Authors:  Ana Arbeloa; Heidi Segal; Jean-Emmanuel Hugonnet; Nathalie Josseaume; Lionnel Dubost; Jean-Paul Brouard; Laurent Gutmann; Dominique Mengin-Lecreulx; Michel Arthur
Journal:  J Bacteriol       Date:  2004-03       Impact factor: 3.490

10.  Modification of penicillin-binding protein 5 associated with high-level ampicillin resistance in Enterococcus faecium.

Authors:  M Ligozzi; F Pittaluga; R Fontana
Journal:  Antimicrob Agents Chemother       Date:  1996-02       Impact factor: 5.191
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  3 in total

1.  Heterogeneity of methicillin-resistant Staphylococcus aureus strains (MRSA) characterized by flow cytometry.

Authors:  Tomasz Jarzembowski; Katarzyna Wiśniewska; Agnieszka Jóźwik; Jacek Witkowski
Journal:  Curr Microbiol       Date:  2009-03-28       Impact factor: 2.188

2.  Flow cytometry approach study of Enterococcus faecalis vancomycin resistance by detection of Vancomycin@FL binding to the bacterial cells.

Authors:  Tomasz Jarzembowski; Agnieszka Jóźwik; Katarzyna Wiśniewska; Jacek Witkowski
Journal:  Curr Microbiol       Date:  2010-04-02       Impact factor: 2.188

3.  A Fluorescent Teixobactin Analogue.

Authors:  Michael A Morris; Melody Malek; Mohammad H Hashemian; Betty T Nguyen; Sylvie Manuse; Kim Lewis; James S Nowick
Journal:  ACS Chem Biol       Date:  2020-02-26       Impact factor: 5.100
  3 in total

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