Literature DB >> 18574543

The full-length mu-opioid receptor: a conformational study by circular dichroism in trifluoroethanol and membrane-mimetic environments.

Isabelle Muller1, Valérie Sarramégna, Marie Renault, Vincent Lafaquière, Sarra Sebai, Alain Milon, Franck Talmont.   

Abstract

The secondary structure content of the recombinant human mu-opioid receptor (HuMOR) solubilized in trifluoroethanol (TFE) and in detergent micelles was investigated by circular dichroism. In both conditions, this G protein-coupled receptor adopts a characteristic alpha-helical structure, with minima at 208 and 222 nm as observed in the circular dichroism spectra. After deconvolution of spectra, the alpha-helix contents were estimated to be in the range of 50% in TFE and in sodium dodecyl sulfate at pH 6. These values are in accordance with the predicted secondary structure content determined for the mu-opioid receptor. A pH-dependent effect was observed on the secondary structure of the receptor solubilized in detergents, which demonstrates the essential role of ionic and hydrophobic interactions on the secondary structure. Circular dichroism spectra of EGFP-HuMOR, a fusion protein between the enhanced green fluorescent protein (EGFP) and the mu-opioid receptor, and EGFP solubilized in TFE were also analyzed as part of this study.

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Year:  2008        PMID： 18574543     DOI： 10.1007/s00232-008-9112-x

Source DB:  PubMed          Journal:  J Membr Biol        ISSN： 0022-2631            Impact factor:   1.843


  59 in total

1.  Expression and spectroscopic characterization of a large fragment of the mu-opioid receptor.

Authors:  Aaron Kerman; Vettai S Ananthanarayanan
Journal:  Biochim Biophys Acta       Date:  2004-11-04

2.  Chaperoning of insertion of membrane proteins into lipid bilayers by hemifluorinated surfactants: application to diphtheria toxin.

Authors:  Sergiy S Palchevskyy; Yevgen O Posokhov; Blandine Olivier; Jean-Luc Popot; Bernard Pucci; Alexey S Ladokhin
Journal:  Biochemistry       Date:  2006-02-28       Impact factor: 3.162

3.  The molecular structure of green fluorescent protein.

Authors:  F Yang; L G Moss; G N Phillips
Journal:  Nat Biotechnol       Date:  1996-10       Impact factor: 54.908

4.  Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding.

Authors:  H Kiefer; J Krieger; J D Olszewski; G Von Heijne; G D Prestwich; H Breer
Journal:  Biochemistry       Date:  1996-12-17       Impact factor: 3.162

Review 5.  GPCR agonists and antagonists in the clinic.

Authors:  Joel D A Tyndall; Radhika Sandilya
Journal:  Med Chem       Date:  2005-07       Impact factor: 2.745

6.  The conformation of the cytoplasmic helix 8 of the CB1 cannabinoid receptor using NMR and circular dichroism.

Authors:  Gregory Choi; Jianxin Guo; Alexandros Makriyannis
Journal:  Biochim Biophys Acta       Date:  2005-02-01

7.  Conformation of a double-membrane-spanning fragment of a G protein-coupled receptor: effects of hydrophobic environment and pH.

Authors:  Aaron Kerman; V S Ananthanarayanan
Journal:  Biochim Biophys Acta       Date:  2007-01-28

8.  Expression and purification of milligram levels of inactive G-protein coupled receptors in E. coli.

Authors:  Steven E Bane; Javier E Velasquez; Anne Skaja Robinson
Journal:  Protein Expr Purif       Date:  2006-11-06       Impact factor: 1.650

9.  Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1.

Authors:  Jean-Louis Baneres; Aimée Martin; Pierre Hullot; Jean-Pierre Girard; Jean-Claude Rossi; Joseph Parello
Journal:  J Mol Biol       Date:  2003-06-13       Impact factor: 5.469

10.  Solution and solid state conformation of the human EGF receptor transmembrane region.

Authors:  A C Rigby; C W Grant; G S Shaw
Journal:  Biochim Biophys Acta       Date:  1998-05-28
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  6 in total

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Journal:  Acta Biomater       Date:  2013-06-14       Impact factor: 8.947

2.  Characterization of an engineered water-soluble variant of the full-length human mu opioid receptor.

Authors:  Jin Xi; Jie Xiao; Jose Manuel Perez-Aguilar; Jinglei Ping; A T Charlie Johnson; Jeffery G Saven; Renyu Liu
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3.  Denatured G-protein coupled receptors as immunogens to generate highly specific antibodies.

Authors:  Franck Talmont; Lionel Moulédous; Jérôme Boué; Catherine Mollereau; Gilles Dietrich
Journal:  PLoS One       Date:  2012-09-27       Impact factor: 3.240

4.  A computationally designed water-soluble variant of a G-protein-coupled receptor: the human mu opioid receptor.

Authors:  Jose Manuel Perez-Aguilar; Jin Xi; Felipe Matsunaga; Xu Cui; Bernard Selling; Jeffery G Saven; Renyu Liu
Journal:  PLoS One       Date:  2013-06-14       Impact factor: 3.240

5.  Improved in Vitro Folding of the Y2 G Protein-Coupled Receptor into Bicelles.

Authors:  Peter Schmidt; Brian J Bender; Anette Kaiser; Khushboo Gulati; Holger A Scheidt; Heidi E Hamm; Jens Meiler; Annette G Beck-Sickinger; Daniel Huster
Journal:  Front Mol Biosci       Date:  2018-01-17

6.  Insights on Structure and Function of a Late Embryogenesis Abundant Protein from Amaranthus cruentus: An Intrinsically Disordered Protein Involved in Protection against Desiccation, Oxidant Conditions, and Osmotic Stress.

Authors:  Alma L Saucedo; Eric E Hernández-Domínguez; Luis A de Luna-Valdez; Angel A Guevara-García; Abraham Escobedo-Moratilla; Esaú Bojorquéz-Velázquez; Federico Del Río-Portilla; Daniel A Fernández-Velasco; Ana P Barba de la Rosa
Journal:  Front Plant Sci       Date:  2017-04-07       Impact factor: 5.753
  6 in total

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