| Literature DB >> 18574321 |
Jing Jing1, Weichao Ren, Xijing Chen, Yue Wang, Qiaoling Yu, Guangji Wang, Andrew K Davey, Jiping Wang, Ning Jing.
Abstract
To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LC-MS total ion chromatogram (TIC). Taken together with the susceptible nature to beta-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.Entities:
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Year: 2008 PMID： 18574321 DOI： 10.2133/dmpk.23.175
Source DB: PubMed Journal: Drug Metab Pharmacokinet ISSN： 1347-4367 Impact factor: 3.614