BACKGROUND: Nonasthmatic eosinophilic bronchitis (EB) has emerged as a useful tool to study the structural and inflammatory mechanisms of airway hyperresponsiveness (AHR) in asthma. We have previously shown that vascular remodeling and reticular basement membrane (RBM) thickening are present in EB. However, it is not known whether other features of structural remodeling including increased airway smooth muscle (ASM) mass, matrix deposition, and glandular hyperplasia are also present in EB. OBJECTIVES: We sought to determine whether structural remodeling occurs in EB and is associated with AHR and airflow limitation. METHODS: Forty-two patients with asthma, 21 patients with EB, and 19 healthy volunteers were recruited. ASM area, RBM thickness, collagen 3 deposition, glandular area, mast cells, and granulocytes were assessed in bronchial biopsy samples. RESULTS: Nonasthmatic eosinophilic bronchitis and asthma were associated with a significant increase in ASM mass and RBM thickness compared with healthy subjects. In contrast, we did not observe any significant differences in collagen 3 deposition in the lamina propria and ASM or the % area of glands in the lamina propria. Univariate analysis demonstrated that mast cell numbers in the ASM were the only feature of remodeling associated with AHR (beta = -0.51; P = .004). Stepwise linear regression revealed that a combination of mast cell numbers in the ASM (beta = -0.43) and disease duration (beta = -0.25; model-adjusted R(2) = 0.26; P = .027) best modeled AHR. CONCLUSION: Mast cell localization to the ASM bundle, but not structural remodeling of the airway wall, is associated with AHR in asthma.
BACKGROUND:Nonasthmatic eosinophilic bronchitis (EB) has emerged as a useful tool to study the structural and inflammatory mechanisms of airway hyperresponsiveness (AHR) in asthma. We have previously shown that vascular remodeling and reticular basement membrane (RBM) thickening are present in EB. However, it is not known whether other features of structural remodeling including increased airway smooth muscle (ASM) mass, matrix deposition, and glandular hyperplasia are also present in EB. OBJECTIVES: We sought to determine whether structural remodeling occurs in EB and is associated with AHR and airflow limitation. METHODS: Forty-two patients with asthma, 21 patients with EB, and 19 healthy volunteers were recruited. ASM area, RBM thickness, collagen 3 deposition, glandular area, mast cells, and granulocytes were assessed in bronchial biopsy samples. RESULTS:Nonasthmatic eosinophilic bronchitis and asthma were associated with a significant increase in ASM mass and RBM thickness compared with healthy subjects. In contrast, we did not observe any significant differences in collagen 3 deposition in the lamina propria and ASM or the % area of glands in the lamina propria. Univariate analysis demonstrated that mast cell numbers in the ASM were the only feature of remodeling associated with AHR (beta = -0.51; P = .004). Stepwise linear regression revealed that a combination of mast cell numbers in the ASM (beta = -0.43) and disease duration (beta = -0.25; model-adjusted R(2) = 0.26; P = .027) best modeled AHR. CONCLUSION: Mast cell localization to the ASM bundle, but not structural remodeling of the airway wall, is associated with AHR in asthma.
Authors: Laurent Benayoun; Anne Druilhe; Marie-Christine Dombret; Michel Aubier; Marina Pretolani Journal: Am J Respir Crit Care Med Date: 2003-01-16 Impact factor: 21.405
Authors: Christopher E Brightling; Peter Bradding; Fiona A Symon; Stephen T Holgate; Andrew J Wardlaw; Ian D Pavord Journal: N Engl J Med Date: 2002-05-30 Impact factor: 91.245
Authors: Mike Berry; Angela Morgan; Dominick E Shaw; Deborah Parker; Ruth Green; Christopher Brightling; Peter Bradding; Andrew J Wardlaw; Ian D Pavord Journal: Thorax Date: 2007-03-13 Impact factor: 9.139
Authors: Finn Rasmussen; D Robin Taylor; Erin M Flannery; Jan O Cowan; Justina M Greene; G Peter Herbison; Malcolm R Sears Journal: Pediatr Pulmonol Date: 2002-09
Authors: Y Obase; T Shimoda; T Kawano; S Saeki; S Tomari; K Izaki; C Fukushima; H Matsuse; S Kohno Journal: Allergy Date: 2003-03 Impact factor: 13.146
Authors: S Siddiqui; S Gupta; G Cruse; P Haldar; J Entwisle; S Mcdonald; P J Whithers; S V Hainsworth; H O Coxson; C Brightling Journal: Allergy Date: 2009-02-11 Impact factor: 13.146
Authors: Stephen T Holgate; Hasan S Arshad; Graham C Roberts; Peter H Howarth; Philipp Thurner; Donna E Davies Journal: Clin Sci (Lond) Date: 2009-12-23 Impact factor: 6.124
Authors: R Saunders; A Sutcliffe; D Kaur; S Siddiqui; F Hollins; A Wardlaw; P Bradding; C Brightling Journal: Clin Exp Allergy Date: 2009-09-07 Impact factor: 5.018
Authors: D Kaur; F Hollins; R Saunders; L Woodman; A Sutcliffe; G Cruse; P Bradding; C Brightling Journal: Clin Exp Allergy Date: 2009-12-16 Impact factor: 5.018
Authors: Jeroen J W Liesker; Nick H Ten Hacken; Mieke Zeinstra-Smith; Steven R Rutgers; Dirkje S Postma; Wim Timens Journal: Int J Chron Obstruct Pulmon Dis Date: 2009-04-15