PRIMARY OBJECTIVE: To determine if plasma levels of 24S-hydroxycholesterol, the primary catabolite of brain cholesterol, provide a measure of axonal damage in acute brain trauma. RESEARCH DESIGN: Determination of plasma 24S-hydroxycholesterol in a series of persons admitted to an intensive care unit for treatment of closed head injury. METHODS AND PROCEDURES: Levels of 24-S-hydroxycholesterol, 27-hydroxycholesterol, lathosterol and total cholesterol were measured in peripheral blood from 38 persons from 14-55 years of age treated by craniotomy and ventriculostomy for intractable intracerebral hypertension. Severity of brain injury was estimated by the Glasgow Coma Scale (range = 3-13, median = 6 points) and overall injury by the Injury Severity Scale (range = 10-48, median = 29). All subjects were intubated and anaesthetized with intravenous propofol. Plasma sterol levels were compared with those of age-matched control subjects. OUTCOMES AND RESULTS: There was no significant increase in plasma 24-S-hydroxycholesterol in subjects with head injury, but measures of peripheral cholesterol synthesis were markedly reduced as compared with values for age-matched normal control subjects. CONCLUSION: Plasma 24S-hydroxycholesterol levels do not change with severe closed head injury.
PRIMARY OBJECTIVE: To determine if plasma levels of 24S-hydroxycholesterol, the primary catabolite of brain cholesterol, provide a measure of axonal damage in acute brain trauma. RESEARCH DESIGN: Determination of plasma 24S-hydroxycholesterol in a series of persons admitted to an intensive care unit for treatment of closed head injury. METHODS AND PROCEDURES: Levels of 24-S-hydroxycholesterol, 27-hydroxycholesterol, lathosterol and total cholesterol were measured in peripheral blood from 38 persons from 14-55 years of age treated by craniotomy and ventriculostomy for intractable intracerebral hypertension. Severity of brain injury was estimated by the Glasgow Coma Scale (range = 3-13, median = 6 points) and overall injury by the Injury Severity Scale (range = 10-48, median = 29). All subjects were intubated and anaesthetized with intravenous propofol. Plasma sterol levels were compared with those of age-matched control subjects. OUTCOMES AND RESULTS: There was no significant increase in plasma 24-S-hydroxycholesterol in subjects with head injury, but measures of peripheral cholesterol synthesis were markedly reduced as compared with values for age-matched normal control subjects. CONCLUSION: Plasma 24S-hydroxycholesterol levels do not change with severe closed head injury.
Authors: D Lütjohann; A Papassotiropoulos; I Björkhem; S Locatelli; M Bagli; R D Oehring; U Schlegel; F Jessen; M L Rao; K von Bergmann; R Heun Journal: J Lipid Res Date: 2000-02 Impact factor: 5.922
Authors: Gloria Lena Vega; Myron F Weiner; Anne M Lipton; Klaus Von Bergmann; Dieter Lutjohann; Carol Moore; Doris Svetlik Journal: Arch Neurol Date: 2003-04
Authors: Frank P Zemlan; Edward C Jauch; J Jeffery Mulchahey; S Prasad Gabbita; William S Rosenberg; Samuel G Speciale; Mario Zuccarello Journal: Brain Res Date: 2002-08-23 Impact factor: 3.252
Authors: Stefan Holdenrieder; Dieter Lütjohann; Sandra Geiger; Klaus von Bergmann; Petra Stieber; Gerhard F Hamann Journal: Neurosci Lett Date: 2004-09-23 Impact factor: 3.046