Effects of early changes in organ dysfunctions on the outcomes of critically ill patients in need of renal replacement therapy.

INTRODUCTION: Acute kidney injury usually develops in critically ill patients in the context of multiple organ dysfunctions.
OBJECTIVE: To evaluate the effect of changes in associated organ dysfunctions over the first three days of renal replacement therapy on the outcomes of patients with acute kidney injury.
METHODS: Over a 19-month period, we evaluated 260 patients admitted to the intensive care units of three tertiary-care hospitals who required renal replacement therapy for > 48 h. Organ dysfunctions were evaluated by SOFA score (excluding renal points) on the first (D1) and third (D3) days of renal replacement therapy. Absolute (A-SOFA) and relative (Delta-SOFA) changes in SOFA scores were also calculated.
RESULTS: Hospital mortality rate was 75%. Organ dysfunctions worsened (A-SOFA>0) in 53%, remained unchanged (A-SOFA=0) in 17% and improved (A-SOFA<0) in 30% of patients; and mortality was lower in the last group (80% vs. 84% vs. 61%, p=0.003). SOFA on D1 (p<0.001), SOFA on D3 (p<0.001), A-SOFA (p=0.019) and Delta-SOFA (p=0.016) were higher in non-survivors. However, neither A-SOFA nor Delta-SOFA discriminated survivors from non-survivors on an individual basis. Adjusting for other covariates (including SOFA on D1), A-SOFA and Delta-SOFA were associated with increased mortality, and patients in whom SOFA scores worsened or remained unchanged had poorer outcomes.
CONCLUSIONS: In addition to baseline values, early changes in SOFA score after the start of renal replacement therapy were associated with hospital mortality. However, no prognostic score should be used as the only parameter to predict individual outcomes.

INTRODUCTION

Acute kidney injury (AKI) occurs frequently in critically ill patients, and despite advances in intensive care and renal replacement therapies (RRT) in recent years, mortality rates remain exceedingly high.1–5 Many predictive factors for increased mortality have been identified in recent years, including older age, the need for RRT itself, the severity of comorbidities and associated organ failures.1–4,6

AKI usually develops in critically ill patients in the context of multiple organ dysfunctions.3 Organ dysfunction scores are routinely used to evaluate these states in critically ill patients. Although no single characteristic or prognostic score should be used as only parameter to predict outcome on individual basis, the sequential evaluation of changes in organ dysfunctions during the first days of admission has been shown to refine outcome prediction in patients admitted to the intensive care unit (ICU).7–9 However, studies focusing on this topic in patients with AKI requiring RRT are both scant and limited by a retrospective design and relatively small sample size.10,11 The aim of the present study was to evaluate the effect of changes in associated organ dysfunctions over the first three days of RRT on the outcomes of a large cohort of patients admitted to the ICU with AKI.

METHODS

From December 2004 to May 2006, a prospective cohort study was performed at the medical-surgical ICU of three tertiary-care hospitals. Since this study did not interfere with clinical decisions related to patients’ care, the Institutional Review Board approved it and waived the need for informed consent in all centers.

Study Participants, Data Collection and Definitions

During the study period, every patient with AKI or acute on chronic kidney injury in need of RRT was evaluated. AKI was classified according to the RIFLE criteria12 at the time of initiation of RRT. Patients with end-stage renal disease requiring chronic dialysis, those with non-renal indications for RRT and those who received RRT in the ICU for < 48 h or had an ICU stay < 48 h were not considered. For patients with readmissions, only the first admission was considered. Decisions to start, change the method, and cease RRT were made by the nephrologist and the intensivist responsible for the patient on an individual basis. Prescribed RRT modes were daily conventional dialysis, daily extended dialysis and continuous RRT (CRRT), taking into consideration the patient’s hemodynamic status.4 CRRT was employed for patients receiving vasoactive drugs and for those with potential for hemodynamic instability. Oliguria was defined as urine output <400 ml/day.

Associated acute organ dysfunctions were evaluated by calculating the Sequential Organ Failure Assessment (SOFA) score,13 excluding renal points. For sedated patients, the Glasgow Coma Scale (GCS) prior to sedation was used.14 SOFA scores were determined on the first (D1) and on the third (D3) days of RRT. The absolute (A-SOFA) and relative (Δ-SOFA) changes in SOFA scores over the first three days of RRT were determined as follows: A-SOFA = SOFA on D3 – SOFA on D1; and Δ-SOFA = (SOFA on D3 – SOFA on D1) / SOFA on D1. Patients with missing data for the calculation of SOFA scores on D1 or D3 were also excluded from the analysis. Patients were classified according to the A-SOFA into three categories: “improved” (A-SOFA < 0), “remained unchanged” (A-SOFA = 0) and “worsened” (A-SOFA > 0). In addition, the following data were collected: age, gender, the Simplified Acute Physiology Score (SAPS) II15 at ICU admission, previous health status,16 comorbidities,17 main reasons for ICU admission, pre-morbid renal function, need of mechanical ventilation and vasopressors for more than 24 h and contributing factors for AKI. The Charlson Comorbidity Index was used in the measurement of comorbidities.17 Sepsis was diagnosed using the criteria developed at the American College of Chest Physicians/Society of Critical Care Medicine consensus conference,18 and infection was diagnosed using criteria from the Centers for Diseases Control.19 Hospital mortality was the end-point of interest. End-of-life decisions (to withhold or withdraw life-support treatments) were made in regard to patients who did not recover from the acute illness despite full-code management.

Data Presentation and Statistical Analysis

Standard descriptive statistics were used to describe the study population. Continuous variables are presented as mean ± standard deviation or median (25%–75% interquartile range). Univariate and multivariate logistic regression analyses were used to identify factors associated with hospital mortality. Linearity between continuous variables and the dependent variable was evaluated using locally weighted scatterplot smoothing (LOWESS).20 Variables yielding P values <0.2 by univariate analysis and variables considered clinically important were entered in a forward multivariate logistic regression analysis. Results of both uni- and multivariate analyses were summarized by estimating odds ratios (OR) and respective 95% confidence intervals (CI). Possible interactions and colinearity were tested. The area under the receiver operating characteristic curve (AROC) was used to evaluate the ability of the models to discriminate between patients who lived and those who died (discrimination).21 The Hosmer-Lemeshow goodness-of-fit test was used to evaluate the model calibration.20 A two-tailed P < 0.05 was considered statistically significant.

RESULTS

Characterization of the Study Population

A total of 429 patients were admitted to the ICUs and required RRT during the study period. Of these patients, 71 (17%) received chronic dialysis for end-stage renal disease, 68 (16%) received RRT in the ICU for < 48 h and 30 (7%) had missing data for the calculation of SOFA scores; as a result, all 169 were excluded from the study. Among patients who received RRT and stayed in the ICU for < 48 h, six (9%) survived and were discharged to the wards. No patient received RRT for non-renal indication. Therefore, the remaining 260 (61%) patients constituted the study population. The main characteristics of patients are depicted in Table 1. The most frequent reasons for ICU admission were sepsis (n=162, 62%), postoperative complications (n=28, 11%), cardiovascular complications (n=25, 10%), AKI (n=10, 4%), trauma (n=8, 3%), neurological complications (n=8, 3%) and miscellaneous (n=19, 7%). Patients were admitted to the ICU at a median of 0 (0–2) days after hospital admission.

Table 1

Main demographic and clinical characteristics of patientsa

Variables	All patients (n=260)	A-SOFA < 0 [n=79 (30)]	A-SOFA = 0 [n=44 (17)]	A-SOFA > 0 [n=137 (53)]	p-valueb
Age (years)	72.9±14.8	70.7±17.1	74.8±15.2	73.5±13.2	0.265
Male sex	135 (52)	34 (43)	29 (66)	72 (53)	0.051c
Hospital days prior to ICU admission (n)	0 (0 – 2)	0 (0 – 2)	0 (0 – 9)	0 (0 – 2)	0.713
Medical admission	209 (80)	68 (86)	39 (89)	102 (75)	0.037
Poor chronic health status (Knaus C or D)	114 (44)	32 (41)	22 (50)	60 (44)	0.596
Charlson Comorbidity Index (points)	3 (1 – 4)	2 (1 – 4)	3 (2 – 5)	3 (1 – 4)	0.149
Charlson Comorbidity Index >1 point	222 (85)	63 (80)	41 (93)	118 (86)	0.121
Body mass index (Kg/m2)	26.0±5.0	26.0±4.6	25.5±3.8	26.2±5.6	0.741
SAPS II (ICU admission, points)	47.5±10.6	46.5±11.4	48.6±9.7	47.7±10.4	0.551
SOFA on day 1 of RRT (except renal points)	5.7±3.1	6.3±3.0	5.5±3.6	5.3±2.9	0.073d
SOFA on day 3 of RRT (except renal points)	6.4±3.5	4.1±3.0	5.5±3.6	8.0±2.9	<0.001c,ddd,eee
A-SOFA (SOFA D3 – SOFA D1) (points)	1 (−1 – 2)	−2 (−3 – −1)	0 (0 – 0)	2 (1 – 4)	<0.001ccc,ddd,eee
Δ-SOFA (SOFA D3 – SOFA D1 / SOFA D1)	0.1 (−1.4 – 0.3)	−0.3 (0.5 – −0.2)	0 (0 – 0)	0.3 (0.2 – 0.8)	<0.001ccc,ddd,eee
RIFLE categories
Risk	68 (26)	23 (29)	8 (18)	37 (27)	0.676
Injury	65 (25)	18 (23)	11 (25)	36 (26)
Failure	127 (49)	38 (48)	25 (57)	64 (47)
Chronic kidney injury	88 (34)	29 (37)	20 (46)	39 (29)	0.095e
RRT at the first day of ICU	91 (35)	30 (38)	15 (34)	46 (34)	0.800
Oliguria	72 (29)	29 (30)	11 (25)	40 (29)	0.812
Sepsis	200 (77)	66 (84)	33 (75)	101 (74)	0.243
Mechanical ventilation	210 (81)	62 (79)	31 (71)	117 (85)	0.075e
Vasopressors	186 (71)	53 (67)	28 (64)	105 (77)	0.144
Length of ICU stay (days)	19 (10 – 37)	19 (13 – 37)	23 (11 – 38)	19 (9 – 35)	0.477
Length of hospital stay (days)	26 (14 – 50)	31 (16 – 52)	30 (16 – 66)	22 (11 – 45)	0.122d
Decision to withhold/withdraw treatment	43 (17)	10 (13)	8 (20)	25 (19)	0.524
ICU mortality	185 (71)	44 (56)	34 (77)	107 (75)	0.001c,ddd
Hospital mortality	194 (75)	48 (61)	37 (84)	109 (80)	0.003c,dd

Results expressed as mean ±SD, median (IQR), n (%).

Comparison among different categories of changes in associated organ failures based on A-SOFA.

p<0.05

p<0.01

p<0.001 for comparisons between patients in whom organ failures improved (A-SOFA < 0) or remained unchanged (A-SOFA = 0).

p<0.05

p<0.01

p<0.001 for comparisons between patients in whom organ failures improved (A-SOFA < 0) or worsened (A-SOFA > 0).

p<0.05

p<0.01

p<0.001 for comparisons between patients in whom organ failures remained unchanged (A-SOFA = 0) or worsened (A-SOFA > 0).

SD = standard deviation, IQR = interquartile range; ICU=intensive care unit; SAPS=Simplified Acute Physiology Score; SOFA=Sequential Organ Failure Assessment; RRT = renal replacement therapy

The main contributing factors for AKI were sepsis (77%), ischemia/shock (67%), contrast/nephrotoxins (29%), major surgeries (22%), rhabdomyolysis (5%), urinary tract obstruction (4%) and other reasons (5%) (a patient could have more than one reason for AKI). CRRT was the initial modality of RRT in 222 (85%) patients. The median time to the start of RRT was three (0–8) days after ICU admission. Ninety-one (35%) patients received RRT the first day in the ICU, and 159 (65%) did thereafter.

The median SOFA score (except renal points) on D1 was six (4–8) points and on D3 was seven (4–9) points. The following organ dysfunctions (> 1 point in each domain) were observed on D1: respiratory (n=207, 80%), cardiovascular (n=189, 73%), hematological (n=120, 46%), hepatic (n=45, 17%) and neurological (n=19, 7%). The median A-SOFA was one (−1 – 2) point, and the median Δ-SOFA was 0.1 (−1.4 – 0.3). According to the A-SOFA, organ dysfunctions improved in 79 (30%), remained unchanged in 44 (17%) and worsened in 137 (53%) patients. Comparisons among the categories of A-SOFA scores are depicted in Table 1. Baseline (D1) organ dysfunctions were more severe in “improved” patients. “Worsened” patients had a lower frequency of medical admission. In general, there were not important differences in patients’ characteristics among the groups (Table 1).

Outcome analysis

The overall ICU and hospital mortality rates were 71% and 75%, respectively. The main outcome data for patients are presented in Table 1. Non-survivors were older than survivors (75.4±12.9 vs. 65.5±17.6 years, P<0.001) and had higher Charlson comorbidity scores [3 (2 – 4) vs. 2 (0 – 4), P=0.023]. As is predictable, SAPS II (49.2±9.7 vs. 42.5±11.5, P<0.001), SOFA D1 [6 (4 – 8) vs. 5 (2 – 7), P=0.011] and SOFA D3 [7 (5 – 9) vs. 5 (1 – 7), P<0.001] scores were higher in decedents than in survivors. A-SOFA [1 (0 – 2) vs. 0 (−2 – 0), P=0.019] and Δ-SOFA [0.11 (0.02 – 0.38) vs. 0 (−0.33 – 0.25), P=0.016] were also higher in non-survivors (Figures 1a and 1b). However, neither the SAPS II score [AROC = 0.69 (95% CI = 0.61 – 0.76)], the A-SOFA [AROC = 0.60 (95% CI = 0.52 – 0.68)] nor the Δ-SOFA [AROC = 0.60 (95% CI = 0.52 – 0.68)] discriminate survivors from non-survivors on an individual basis. In Figure 2, the mortality rates for the different possibilities of the A-SOFA according to the different degrees of severity of organ failures at baseline (SOFA-D1) are depicted. The values corresponding to the terciles of distribution of the SOFA score on D1 (SOFA D1) were used to establish a cut-off point to stratify the patients into three groups. The hospital mortality was significantly higher in patients in whom organ dysfunction worsened or remained unchanged.

Figure 1

Boxplot graphics representing the comparisons of absolute (A-SOFA) [Figure 1a] and relative (Δ-SOFA) [Figure 1b] changes in associated organ failures between survivors and non-survivors. P-values refer to the Mann-Whitney U test.

Figure 2

Hospital mortality rates (y-axis) according to the degree of severity of organ failures on Day 1. The values corresponding to the terciles of distribution of the SOFA score on Day 1 (SOFA D1) (x-axis) were used to establish a cut-off point for stratifying the patients into three groups (columns). Patients in whom associated organ failures improved (A-SOFA < 0), remained unchanged (A-SOFA = 0) and worsened (A-SOFA > 0) are represented in gray, square-lined and black columns, respectively. a) P = 0.05; b) P = 0.19; c) P = 0.04.

The results of univariate analysis of predictive factors for increased hospital mortality are depicted in Table 2. Older age, poor chronic health status, presence of comorbidities, body mass index, SOFA D1, A-SOFA, Δ-SOFA and A-SOFA categories were associated with a worse outcome. Three multivariate analyses were performed, entering either A-SOFA, Δ-SOFA or A-SOFA categories in separate models (Table 3). The main independent outcome predictors were older age, the presence of comorbidities, poor functional capacity, baseline organ dysfunctions (SOFA D1) and all forms of evaluation of changes in organ dysfunctions. Patients in whom organ dysfunction worsened or remained unchanged had a worse outcome adjusting for other significant covariates. All multivariate models had both good calibration and discrimination (Table 3). Sepsis, acute on chronic kidney dysfunction and oliguria were forced into the final models and were not selected. The SAPS II score was also entered into the final models (OR=1.02 (0.98–1.06), p=0.267 in the model which included the A-SOFA; OR=1.02 (0.98–1.06), p=0.241 in the model which included the Δ-SOFA; OR=1.02 (0.98–1.06), p=0.258 in the model which included the A-SOFA categories). The SAPS II score was not selected in all of them, and in general, the effects of the other covariates remained unchanged.

Table 2

Univariate analysis of factors associated with increased hospital mortality (n=260)

Variables	Mortality (%)	Odds-ratios (95% CI)	p-value
Age (years)	-	1.04 (1.02 – 1.06)	<0.001
Gender
Female	71	1.00	0.282
Male	78	1.42 (0.81 – 2.48)
Poor chronic health status (Knaus C or D)
No	62	1.00	<0.001
Yes	90	5.66 (2.79 – 11.47)
Charlson comorbidity index (points)
0	50	1.00	<0.001
≥ 1	79	3.72 (1.83 – 7.59)
Type of admission
Surgical	67	1.00	0.202
Medical	77	1.63 (0.84 – 3.17)
Days prior to ICU admission (Ln)	-	1.058 (0.90 – 1.30)	0.426
Mechanical ventilation
No	74	1.00	0.999
Yes	75	1.04 (0.52 – 2.11)
Sepsis
No	70	1.00	0.443
Yes	76	1.36 (0.72 – 2.58)
Vasopressors
No	68	1.00	0.135
Yes	76	1.62 (0.91 – 2.90)
Acute on chronic kidney injury
No	74	1.00	0.801
Yes	76	1.13 (0.62 – 2.05)
RRT on the first day in ICU
No	78	1.00	0.107
Yes	68	0.60 (0.34 – 1.06)
Oliguria
No	73	1.00	0.425
Yes	79	1.37 (0.72 – 2.59)
SOFA D1	-	1.13 (1.03 – 1.25)	0.009
A-SOFA	-	1.13 (1.01 – 1.26)	0.038
Δ-SOFA	-	1.26 (0.86 – 1.84)	0.231
A-SOFA categories
Improved (< 0)	61	1.00	0.003
Remained unchanged (= 0)	84	3.41 (1.35 – 8.61)
Worsened (> 0)	80	2.51 (1.36 – 4.64)

CI = confidence interval; ICU = intensive care unit; SOFA = Sequential Organ Failure Assessment.

Table 3

Multivariate analyses of factors associated with increased hospital mortality (n=260)

Variables	Odds-ratios (95% CI)	P value
Model including A-SOFAa
Age (years)	1.03 (1.01 – 1.06)	0.021
Charlson comorbidity index (points)
0	1.00
≥ 1	3.84 (1.57 – 9.39)	0.003
Poor chronic health status (Knaus C or D)
No	1.00
Yes	3.37 (1.49 – 7.65)	0.004
Type of admission
Surgical	1.00	0.049
Medical	2.27 (1.00 – 5.13)
SOFA D1	1.32 (1.16 – 1.49)	<0.001
A-SOFA	1.27 (1.10 – 1.46)	0.001
Model including Δ-SOFAb
Age (years)	1.03 (1.00 – 1.06)	0.024
Charlson comorbidity index (points)
0	1.00
≥ 1	3.38 (1.41 – 8.08)	0.006
Poor chronic health status (Knaus C or D)
No	1.00
Yes	3.90 (1.74 – 8.75)	0.001
SOFA D1	1.31 (1.16 – 1.48)	<0.001
Δ-SOFA	1.84 (1.16 – 2.91)	0.009
Model including A-SOFA categoriesc
Age (years)	1.03 (1.00 – 1.06)	0.022
Charlson comorbidity index (points)
0	1.00
≥ 1	3.34 (1.37 – 8.14)	0.008
Poor chronic health status (Knaus C or D)
No	1.00
Yes	3.60 (1.58 – 8.22)	0.002
Type of admission
Surgical	1.00	0.066
Medical	2.15 (0.96 – 4.87)
SOFA D1	1.29 (1.14 – 1.45)	<0.001
A-SOFA categories
Improved (< 0)	1.00
Remained unchanged (= 0)	4.29 (1.45 – 12.72)	0.009
Worsened (> 0)	4.07 (1.91 – 4.87)	<0.001

CI = confidence interval; SOFA = Sequential Organ Failure Assessment.

AROC = 0.81 (95% CI = 0.75–0.87); Hosmer-Lemeshow goodness-of-fit (χ2=4.144; P=0.844).

AROC = 0.72 (95% CI = 0.65–0.80); Hosmer-Lemeshow goodness-of-fit (χ2=8.234; P=0.411).

AROC = 0.73 (95% CI = 0.66–0.80); Hosmer-Lemeshow goodness-of-fit (χ2=6.241; P=0.620).

DISCUSSION

Although changes in organ dysfunctions have already been reported to be associated with outcomes in general ICU patients22, information on this subject in patients with AKI is limited. Patients with AKI have higher mortality rates, especially when RRT is required, and their outcomes are significantly worse than those of other patients.1–5 It has long been known that prognostic scores or even specific renal scores used at ICU admission do not perform well in the AKI population.23,24 In the present multicenter study, we have prospectively investigated the effect of early changes in associated organ dysfunctions on the mortality of a large cohort of critically ill patients with AKI in need of RRT. We employed the SOFA score, a simple, validated and widely used organ dysfunction score for this purpose. Our results demonstrated that, in addition to well-known outcome predictors, such as older age, medical admission, a poor previous chronic health status, comorbidities and the severity of baseline organ dysfunctions, the sequential trend in associated organ dysfunctions over the first three days of RRT was associated with increased hospital mortality. However, it was also demonstrated that these trends in organ dysfunctions as a single parameter were not able to discriminate survivors and non-survivors and that they therefore should not be used to predict outcome on an individual basis.

Previous studies have reported that the outcomes of patients with AKI are worse in the context of multiple organ dysfunctions and that mortality rates increase with the number of organ dysfunctions.1,4 Organ dysfunctions are an ongoing event changing over the clinical course of ICU patients, and their evaluation at a static point may not always reflect the real patient’s clinical condition. Changes in SOFA scores over the first 24 h or 48 h have been demonstrated to refine outcome prediction in patients with severe sepsis8 and in general ICU patients,7 respectively. Chertow et al.25 reported an enhanced prediction power with the sequential analysis of different prognostic scores, including SOFA score, in the AKI population.

Although it is intuitive that the course of associated organ dysfunctions correlates with outcomes in patients with AKI in need of RRT, to the best of our knowledge only two previous studies have addressed this issue.10,11

Kikuchi et al.11 evaluated 40 patients who underwent different extracorporeal blood purification procedures. However, in addition to patients with AKI receiving RRT (45% of the entire population), they also evaluated patients treated with plasma exchange, bilirubin and endotoxin adsorption. In accord with our results, they demonstrated that although changes in SOFA scores did not discriminate mortality on an individual basis, non-survivors had higher maximum SOFA scores and higher changes in SOFA scores during RRT procedures than survivors.

More recently, Cappi et al.10 retrospectively evaluated 111 patients with AKI in need of RRT. In that study, independent predictors of increased ICU mortality were cardiovascular dysfunction at ICU admission, changes in total SOFA scores over the first 24 h of ICU stay and older age. However, as acknowledged by the authors, the study was limited by its retrospective design and by a relatively small sample size. In addition, many other outcome predictors such as comorbidities and chronic health status, which would be interesting to adjust for, were not evaluated.

In a very elegant study, Levy et al.8 evaluated the impact of changes in total SOFA score and its domains over the first 24 h in 1,034 patients with severe sepsis. They demonstrated that improvements in organ dysfunctions correlated significantly with survival. By contrast, patients in whom organ dysfunctions remained unchanged or worsened presented with comparatively higher 28-day mortality rates. These observations are in agreement with our results. In our study, the multivariate analysis showed four-fold higher odds of hospital mortality in patients with AKI in whom the associated organ failures remained unchanged or worsened in comparison to those in whom organ failures improved. These facts remained significant even when the patients were stratified according to the severity of baseline organ dysfunctions.

Nonetheless, there are some limitations in our study which must be taken into account in the generalization of its results. First, although patients from three tertiary-care centers were included, the study was restricted to a specific geographic area. Moreover, the decision to start RRT was left at the discretion of the attending nephrologist and intensivist. Even though it is reasonable to presume that the criteria employed in this study are similar to those accepted in many ICUs, indications for RRT vary significantly among institutions. Due to the non-interventional nature of the present study, it is not possible to conclude whether changes in organ failures over time can be used to evaluate the response to RRT. In a recent report, Sakr et al.26 have demonstrated the applicability of daily renal SOFA score analysis as an evaluation tool for starch effects in renal function. However, it should be emphasized that no prognostic score, including severity of organ failure scores, can be used as the only parameter in decisions related to the patient’s care. Another criticism to consider is that SOFA scores should ideally have been collected daily while RRT was used. However, the decision to measure changes in SOFA scores on D1 and D3 was made based on the study of Ferreira et al.7 In that study, changes in SOFA score over the first 48 h better predicted mortality than changes observed on the subsequent days did. Finally, the mortality rates of our patients were high. However, our patient population is significantly old (69% of the patients were older than 70 years), 85% had comorbidities and 44% had poor chronic health status. Older age, severity of comorbidities and poor chronic health status are well-known risk factors for increased mortality in critically ill patients.

Our results indicate that early changes in SOFA score after the start of RRT were associated with hospital mortality of patients with AKI. Adjusting for other relevant covariates, including the initial severity of organ dysfunctions, patients in whom SOFA scores worsened or remained unchanged had poorer outcomes. On the other hand, a decrease in SOFA score over the first three days of RRT was associated with improved survival in patients with AKI. In conjunction with clinical judgment, and taking into consideration the patient’s preferences and values, the knowledge of these outcome predictors may be useful in helping physicians make decisions in the care of these patients and in improving discussions of a patient’s prognosis. However, changes in SOFA scores did not discriminate outcomes on an individual basis and should not be used as a single parameter for predicting outcomes and evaluating patients’ response to therapeutic interventions such as RRT.