| Literature DB >> 18565645 |
Hui Liu1, Cheng-Kun Qin, Guo-Qing Han, Hong-Wei Xu, Wan-Hua Ren, Cheng-Yong Qin.
Abstract
We investigated whether HS-1200 has anti-proliferation effects on human hepatoma cells in vitro. Here, chromatin condensation, DNA ladder formation and proteolytic cleavage of poly (ADP-ribose) polymerase (PARP) were observed after treatment of HS-1200, indicating the occurrence of apoptotic cell death, which was associated with up-regulation of Bax, cleaved-caspase-3 and cleaved-caspase-9. Inhibition of caspase-9 rescued HS-1200-induced apoptosis. Furthermore, cells treated with HS-1200 showed a reduction in mitochondrial membrane potential (Deltapsi(m)) and caused cytochrome c release into the cytosol. The results indicated that synthetic chenodeoxycholic acid HS-1200 could induce cell apoptosis in BEL7402 human hepatoma cell line, via a Bax/cytochrome c/caspase-9 independent pathway. This study suggested that HS-1200 is potentially useful as an apoptosis inducer for the treatment of hepatocellular carcinoma.Entities:
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Year: 2008 PMID: 18565645 DOI: 10.1016/j.canlet.2008.05.014
Source DB: PubMed Journal: Cancer Lett ISSN: 0304-3835 Impact factor: 8.679