Two-step purification of low-content cellular protein using protein-imprinted polymers.

We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.