OBJECTIVES: This study was designed to quantify the specific glycosaminoglycans (GAGs) in the midmembranous vocal fold (VF) lamina propria (LP) and to interpret their presence in relation to the known stresses borne by each LP layer. METHODS: GAGs from normal human LP and from both normal and scarred canine LPs were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE). Immunostaining was conducted to give insight into the spatial distribution of each GAG type. RESULTS: Hyaluronan composes roughly 0.64% +/- 0.41% of the human LP as measured relative to tissue total protein. Chondroitin sulfate and/or dermatan sulfate (CS/DS), keratan sulfate, and heparan sulfate chains constitute approximately 23.9% +/- 12.1%, 14.7% +/- 6.1%, and 61.4% +/- 13.6%, respectively, of human LP sulfated GAGs. CONCLUSIONS: Observed CS/DS sulfation patterns imply that versican is a major contributor to human LP CS levels. In addition, examination of LP GAG with respect to gender revealed a significant variation in total levels of CS/DS and a potential difference in the levels of versican relative to decorin and biglycan. In dogs, LP scarring appeared to result in a reduction in hyaluronan and CS/DS. These FACE results were combined with histologic data to update current descriptive models linking LP microstructure with the regional variations in LP loading.
OBJECTIVES: This study was designed to quantify the specific glycosaminoglycans (GAGs) in the midmembranous vocal fold (VF) lamina propria (LP) and to interpret their presence in relation to the known stresses borne by each LP layer. METHODS: GAGs from normal human LP and from both normal and scarred canineLPs were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE). Immunostaining was conducted to give insight into the spatial distribution of each GAG type. RESULTS:Hyaluronan composes roughly 0.64% +/- 0.41% of the human LP as measured relative to tissue total protein. Chondroitin sulfate and/or dermatan sulfate (CS/DS), keratan sulfate, and heparan sulfate chains constitute approximately 23.9% +/- 12.1%, 14.7% +/- 6.1%, and 61.4% +/- 13.6%, respectively, of human LP sulfated GAGs. CONCLUSIONS: Observed CS/DS sulfation patterns imply that versican is a major contributor to humanLP CS levels. In addition, examination of LP GAG with respect to gender revealed a significant variation in total levels of CS/DS and a potential difference in the levels of versican relative to decorin and biglycan. In dogs, LP scarring appeared to result in a reduction in hyaluronan and CS/DS. These FACE results were combined with histologic data to update current descriptive models linking LP microstructure with the regional variations in LP loading.
Authors: Lauren Pruett; Christian Jenkins; Neharika Singh; Katarina Catallo; Donald Griffin Journal: Adv Funct Mater Date: 2021-06-18 Impact factor: 19.924
Authors: Andrew J Bowen; Dale C Ekbom; Danielle Hunter; Stephen Voss; Kathleen Bartemes; Andrew Mearns-Spragg; Michael S Oldenburg; Serban San-Marina Journal: Laryngoscope Investig Otolaryngol Date: 2022-09-24
Authors: Michael Döllinger; James Kobler; David A Berry; Daryush D Mehta; Georg Luegmair; Christopher Bohr Journal: Curr Bioinform Date: 2011 Impact factor: 3.543