BACKGROUND: The increased susceptibility of asthmatics to rhinovirus infection has recently been related to deficient IFN-lambda 1 (IL-29) and IFN-lambda 2/3 (IL-28) production by bronchial epithelial cells and macrophages. OBJECTIVES: Here, we studied IFN-lambda mRNA expression in the airways of stable asthmatics in comparison with healthy subjects and in relation to asthma symptoms, non-invasive parameters of airway inflammation and lung function parameters. METHODS: Airway cells were obtained by sputum induction, in 14 healthy and 35 asthmatic adults and 12 asthmatic school-aged children. IFN-lambda was studied at the mRNA level by quantitative RT-PCR. RESULTS: Asthmatic adults have increased sputum IL-28 mRNA but similar IL-29 mRNA expression in comparison with healthy subjects. In asthmatics, both sputum IL-28 and IL-29 mRNA expression correlate with the sputum CD3 gamma mRNA expression (reflecting infiltrated T cells). IL-28 (but not IL-29) mRNA levels correlate with the relative and absolute number of eosinophils present in the sputum sample. Sputum IL-29 mRNA (but not IL-28) correlates negatively with asthma symptoms in steroid-naive patients and is significantly higher in steroid-treated than in steroid-naive patients. Finally, both IL-28 and IL-29 mRNA levels are higher in asthmatic children than in asthmatic adults. CONCLUSION: Our results show that asthmatic subjects have substantial type III IFN-lambda mRNA levels in the airways. Our data furthermore suggest that IL-29 could have an immunoprotective role in the lower airways.
BACKGROUND: The increased susceptibility of asthmatics to rhinovirus infection has recently been related to deficient IFN-lambda 1 (IL-29) and IFN-lambda 2/3 (IL-28) production by bronchial epithelial cells and macrophages. OBJECTIVES: Here, we studied IFN-lambda mRNA expression in the airways of stable asthmatics in comparison with healthy subjects and in relation to asthma symptoms, non-invasive parameters of airway inflammation and lung function parameters. METHODS: Airway cells were obtained by sputum induction, in 14 healthy and 35 asthmatic adults and 12 asthmatic school-aged children. IFN-lambda was studied at the mRNA level by quantitative RT-PCR. RESULTS: Asthmatic adults have increased sputum IL-28 mRNA but similar IL-29 mRNA expression in comparison with healthy subjects. In asthmatics, both sputum IL-28 and IL-29 mRNA expression correlate with the sputum CD3 gamma mRNA expression (reflecting infiltrated T cells). IL-28 (but not IL-29) mRNA levels correlate with the relative and absolute number of eosinophils present in the sputum sample. Sputum IL-29 mRNA (but not IL-28) correlates negatively with asthma symptoms in steroid-naive patients and is significantly higher in steroid-treated than in steroid-naive patients. Finally, both IL-28 and IL-29 mRNA levels are higher in asthmatic children than in asthmatic adults. CONCLUSION: Our results show that asthmatic subjects have substantial type III IFN-lambda mRNA levels in the airways. Our data furthermore suggest that IL-29 could have an immunoprotective role in the lower airways.
Authors: E Kathryn Miller; Johanna Zea Hernandez; Vera Wimmenauer; Bryan E Shepherd; Diego Hijano; Romina Libster; M Elina Serra; Niranjan Bhat; Juan P Batalle; Yassir Mohamed; Andrea Reynaldi; Andrea Rodriguez; Monica Otello; Nestor Pisapia; Jimena Bugna; Miguel Bellabarba; David Kraft; Silvina Coviello; F Martin Ferolla; Aaron Chen; Stephanie J London; George K Siberry; John V Williams; Fernando P Polack Journal: Am J Respir Crit Care Med Date: 2011-12-01 Impact factor: 21.405
Authors: Mei-Ting Tao; Ya-Ping Xie; Shu-Ping Liu; Hao-Feng Chen; Han Huang; Min Chen; Li-Li Zhong Journal: Zhongguo Dang Dai Er Ke Za Zhi Date: 2017-06
Authors: Nirav R Bhakta; Stephanie A Christenson; Srilaxmi Nerella; Owen D Solberg; Christine P Nguyen; David F Choy; Kyle L Jung; Suresh Garudadri; Luke R Bonser; Joshua L Pollack; Lorna T Zlock; David J Erle; Charles Langelier; Joseph L Derisi; Joseph R Arron; John V Fahy; Prescott G Woodruff Journal: Am J Respir Crit Care Med Date: 2018-02-01 Impact factor: 21.405