BACKGROUND AND OBJECTIVE: We evaluated the endpoint of caries removal with a novel fluorescence-controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy. MATERIALS AND METHODS: For the biochemical analysis 210 carious and 60 caries-free teeth were treated by a fluorescence controlled Er:YAG laser (n=240) or by conventional drilling (n=30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM. RESULTS: Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found. CONCLUSION: The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin.
BACKGROUND AND OBJECTIVE: We evaluated the endpoint of caries removal with a novel fluorescence-controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy. MATERIALS AND METHODS: For the biochemical analysis 210 carious and 60 caries-free teeth were treated by a fluorescence controlled Er:YAG laser (n=240) or by conventional drilling (n=30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM. RESULTS: Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found. CONCLUSION: The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin.
Authors: Walter Raucci-Neto; Carla Raquel Dos Santos; Fabrício Augusto de Lima; Jesus Djalma Pécora; Luciano Bachmann; Regina Guenka Palma-Dibb Journal: Lasers Med Sci Date: 2014-04-23 Impact factor: 3.161
Authors: Nicole R Lamantia; Henry Tom; Kenneth H Chan; Jacob C Simon; Cynthia L Darling; Daniel Fried Journal: Proc SPIE Int Soc Opt Eng Date: 2014-02-18
Authors: William A Fried; Kenneth H Chan; Cynthia L Darling; Donald A Curtis; Daniel Fried Journal: Lasers Surg Med Date: 2019-06-24 Impact factor: 4.025