Literature DB >> 1856292

Microbiological assay for vitamin B12 performed in 96-well microtitre plates.

B P Kelleher1, S D Broin.   

Abstract

A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.

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Year:  1991        PMID： 1856292      PMCID： PMC496801          DOI： 10.1136/jcp.44.7.592

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  6 in total

1.  INVESTIGATIONS INTO THE EUGLENA METHOD FOR THE ASSAY OF THE VITAMIN B12 IN SERUM.

Authors:  B B ANDERSON
Journal:  J Clin Pathol       Date:  1964-01       Impact factor: 3.411

2.  An improved method for the rapid estimation of vitamin B12 in serum.

Authors:  G H SPRAY
Journal:  Clin Sci       Date:  1955-11       Impact factor: 6.124

3.  Status of laboratory testing in the diagnosis of megaloblastic anemia.

Authors:  J Lindenbaum
Journal:  Blood       Date:  1983-04       Impact factor: 22.113

4.  The significance of the serum vitamin B 12 estimation in clinical practice.

Authors:  I J Temperley; D Collery
Journal:  Ir J Med Sci       Date:  1965 Jul-Oct       Impact factor: 1.568

Review 5.  The serum vitamin B12 level: its assay and significance.

Authors:  D L Mollin; B B Anderson; J F Burman
Journal:  Clin Haematol       Date:  1976-10

6.  Use of beta-lactamase to hydrolyse interfering antibiotics in vitamin B12 microbiological assay using Lactobacillus leichmannii.

Authors:  B P Kelleher; J M Scott; S D O'Broin
Journal:  Clin Lab Haematol       Date:  1990
  6 in total
  63 in total

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Journal:  Gut       Date:  1999-09       Impact factor: 23.059

2.  Vitamin B12 status, homocysteine and mortality amongst community-dwelling Irish elders.

Authors:  D J Robinson; C O'Luanaigh; E Tehee; H O'Connell; F Hamilton; A V Chin; R Coen; A M Molloy; J Scott; B A Lawlor; C J Cunningham
Journal:  Ir J Med Sci       Date:  2010-11-13       Impact factor: 1.568

Review 3.  Circulating folate, vitamin B12, homocysteine, vitamin B12 transport proteins, and risk of prostate cancer: a case-control study, systematic review, and meta-analysis.

Authors:  Simon M Collin; Chris Metcalfe; Helga Refsum; Sarah J Lewis; Luisa Zuccolo; George Davey Smith; Lina Chen; Ross Harris; Michael Davis; Gemma Marsden; Carole Johnston; J Athene Lane; Marta Ebbing; Kaare Harald Bønaa; Ottar Nygård; Per Magne Ueland; Maria V Grau; John A Baron; Jenny L Donovan; David E Neal; Freddie C Hamdy; A David Smith; Richard M Martin
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4.  Functional inference of the methylenetetrahydrofolate reductase 677C > T and 1298A > C polymorphisms from a large-scale epidemiological study.

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5.  Intraindividual variation in one-carbon metabolism plasma biomarkers.

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Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2013-08-15       Impact factor: 4.254

6.  Dairy Intakes in Older Irish Adults and Effects on Vitamin Micronutrient Status: Data from the TUDA Study.

Authors:  E Laird; M C Casey; M Ward; L Hoey; C F Hughes; K McCarroll; C Cunningham; J J Strain; H McNulty; A M Molloy
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7.  Microbiological assay on microtitre plates of folate in serum and red cells.

Authors:  S O'Broin; B Kelleher
Journal:  J Clin Pathol       Date:  1992-04       Impact factor: 3.411

8.  Plasma vitamins B2, B6, and B12, and related genetic variants as predictors of colorectal cancer risk.

Authors:  Simone J P M Eussen; Stein Emil Vollset; Steinar Hustad; Øivind Midttun; Klaus Meyer; Ase Fredriksen; Per Magne Ueland; Mazda Jenab; Nadia Slimani; Paolo Boffetta; Kim Overvad; Ole Thorlacius-Ussing; Anne Tjønneland; Anja Olsen; Françoise Clavel-Chapelon; Marie-Christine Boutron-Ruault; Sophie Morois; Cornelia Weikert; Tobias Pischon; Jakob Linseisen; Rudolf Kaaks; Antonia Trichopoulou; Demosthenes Zilis; Michael Katsoulis; Domenico Palli; Valeria Pala; Paolo Vineis; Rosario Tumino; Salvatore Panico; Petra H M Peeters; H Bas Bueno-de-Mesquita; Fränzel J B van Duijnhoven; Guri Skeie; Xavier Muñoz; Carmen Martínez; Miren Dorronsoro; Eva Ardanaz; Carmen Navarro; Laudina Rodríguez; Bethany VanGuelpen; Richard Palmqvist; Jonas Manjer; Ulrika Ericson; Sheila Bingham; Kay-Tee Khaw; Teresa Norat; Elio Riboli
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2010-09-02       Impact factor: 4.254

9.  A Daily Dose of 5 mg Folic Acid for 90 Days Is Associated with Increased Serum Unmetabolized Folic Acid and Reduced Natural Killer Cell Cytotoxicity in Healthy Brazilian Adults.

Authors:  Clovis Paniz; Juliano Felix Bertinato; Maylla Rodrigues Lucena; Eduardo De Carli; Patrícia Mendonça da Silva Amorim; Guilherme Wataru Gomes; Cecília Zanin Palchetti; Maria Stella Figueiredo; Christine M Pfeiffer; Zia Fazili; Ralph Green; Elvira Maria Guerra-Shinohara
Journal:  J Nutr       Date:  2017-07-19       Impact factor: 4.798

10.  Maternal B vitamin status in pregnancy week 18 according to reported use of folic acid supplements.

Authors:  Anne Lise Bjørke-Monsen; Christine Roth; Per Magnus; Øivind Midttun; Roy M Nilsen; Ted Reichborn-Kjennerud; Camilla Stoltenberg; Ezra Susser; Stein Emil Vollset; Per Magne Ueland
Journal:  Mol Nutr Food Res       Date:  2012-09-24       Impact factor: 5.914
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