Andrea T Badillo1, Liping Zhang, Kenneth W Liechty. 1. The Center for Fetal Research at The Children's Hospital of Philadelphia, The University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Abstract
BACKGROUND/ PURPOSE: Stromal progenitor cells (SPC) enhance tissue repair in a variety of injury models. However, the mechanisms by which SPCs facilitate tissue repair remain poorly understood. We hypothesized that SPC-enhanced tissue repair is, in part, because of SPC-mediated recruitment of circulating cells to areas of tissue injury. To test this, we examined the migration of leukocytes in response to SPC in vitro. METHODS: Leukocyte migration was assessed in response to SPC, SPC + transforming growth factor (TGF)-beta1, or SPC + AMD3100 using a Transwell assay system (Corning, distributed by Fisher Scientific, Pittsburgh, PA). Supernatants were collected from lower chambers and analyzed for leukocyte content, leukocyte viability, and stromal-derived growth factor (SDF)-1alpha concentration. RESULTS: Stromal progenitor cells increased leukocyte migration compared to media alone (450 +/- 70 vs 112 +/- 17 cells/microL; P < .05). SPC treatment with TGF-beta1 resulted in a 36% increase in leukocyte migration and correlated with an increase in SDF-1alpha production. Treatment with AMD3100 resulted in inhibition of leukocyte migration. CONCLUSIONS: Stromal progenitor cells promote leukocyte migration, and this appears to be mediated through SDF-1alpha production. The SPC production of SDF-1alpha may be modulated by other cytokines present in the microenvironment during wound healing. Together, these observations provide a potential mechanism by which SPC may augment healing through enhanced recruitment of inflammatory cells and tissue progenitor cells to areas of tissue injury.
BACKGROUND/ PURPOSE: Stromal progenitor cells (SPC) enhance tissue repair in a variety of injury models. However, the mechanisms by which SPCs facilitate tissue repair remain poorly understood. We hypothesized that SPC-enhanced tissue repair is, in part, because of SPC-mediated recruitment of circulating cells to areas of tissue injury. To test this, we examined the migration of leukocytes in response to SPC in vitro. METHODS: Leukocyte migration was assessed in response to SPC, SPC + transforming growth factor (TGF)-beta1, or SPC + AMD3100 using a Transwell assay system (Corning, distributed by Fisher Scientific, Pittsburgh, PA). Supernatants were collected from lower chambers and analyzed for leukocyte content, leukocyte viability, and stromal-derived growth factor (SDF)-1alpha concentration. RESULTS: Stromal progenitor cells increased leukocyte migration compared to media alone (450 +/- 70 vs 112 +/- 17 cells/microL; P < .05). SPC treatment with TGF-beta1 resulted in a 36% increase in leukocyte migration and correlated with an increase in SDF-1alpha production. Treatment with AMD3100 resulted in inhibition of leukocyte migration. CONCLUSIONS: Stromal progenitor cells promote leukocyte migration, and this appears to be mediated through SDF-1alpha production. The SPC production of SDF-1alpha may be modulated by other cytokines present in the microenvironment during wound healing. Together, these observations provide a potential mechanism by which SPC may augment healing through enhanced recruitment of inflammatory cells and tissue progenitor cells to areas of tissue injury.
Authors: Michael S Hu; Zeshaan N Maan; Jen-Chieh Wu; Robert C Rennert; Wan Xing Hong; Tiffany S Lai; Alexander T M Cheung; Graham G Walmsley; Michael T Chung; Adrian McArdle; Michael T Longaker; H Peter Lorenz Journal: Ann Biomed Eng Date: 2014-05-01 Impact factor: 3.934
Authors: L Ciocca; D Donati; S Ragazzini; B Dozza; F Rossi; M Fantini; A Spadari; N Romagnoli; E Landi; A Tampieri; A Piattelli; G Iezzi; R Scotti Journal: Biomed Res Int Date: 2013-09-01 Impact factor: 3.411
Authors: I-Ping Lee; Andrew K Evans; Cissy Yang; Melissa G Works; Vineet Kumar; Zurine De Miguel; Nathan C Manley; Robert M Sapolsky Journal: PLoS One Date: 2014-10-09 Impact factor: 3.240