PURPOSE: We investigated the effect of the duration of vaginal distention on the differential expression of stem cell homing, tissue repair cytokines and cytokine receptors to identify the factors most important for recovery from injury. MATERIALS AND METHODS: A total of 20, 10-week-old virgin Sprague-Dawley rats were divided into 4 groups, including 1, 4 and 6-hour vaginal distention, and anesthetized sham operation. The vagina, bladder, urethra and rectum were harvested immediately after vaginal distention. Real-time polymerase chain reaction was used to determine the relative expression of cytokines and receptors of interest. Mixed models analysis was used to determine associations between expression levels and vaginal distention duration. RESULTS: Positive associations between vaginal distention duration and the urethral expression level were found for 1 of the receptors of monocyte chemotactic protein-3, CCR1 (p = 0.0001) as well as for monocyte chemotactic protein-3 (p = 0.025), CCR5 (p = 0.032) and hypoxia inducible factor-1alpha (p = 0.023). A positive relationship between vaginal distention duration and monocyte chemotactic protein-3 expression was also observed in rectal tissue (p = 0.035). Urethral expression of CCR2, another receptor for monocyte chemotactic protein-3, approached significance (p = 0.066). An inverse relationship between vaginal distention duration and interleukin-8 expression was found in the bladder (p = 0.0008). No association was noted between vaginal distention duration and the expression of stromal derived factor-1, CXCR4, CCR3 and vascular endothelial growth factor in any pelvic organs. CONCLUSIONS: These data support a relationship between vaginal distention duration and the subsequent expression of monocyte chemotactic protein-3 and 1 of its associated receptors, CCR1, in the urethra immediately following vaginal distention. The increase in hypoxia-inducible factor1alpha expression in the urethra with prolonged vaginal distention suggests a limited role of tissue ischemia in the immediate response of pelvic organs to vaginal distention.
PURPOSE: We investigated the effect of the duration of vaginal distention on the differential expression of stem cell homing, tissue repair cytokines and cytokine receptors to identify the factors most important for recovery from injury. MATERIALS AND METHODS: A total of 20, 10-week-old virgin Sprague-Dawley rats were divided into 4 groups, including 1, 4 and 6-hour vaginal distention, and anesthetized sham operation. The vagina, bladder, urethra and rectum were harvested immediately after vaginal distention. Real-time polymerase chain reaction was used to determine the relative expression of cytokines and receptors of interest. Mixed models analysis was used to determine associations between expression levels and vaginal distention duration. RESULTS: Positive associations between vaginal distention duration and the urethral expression level were found for 1 of the receptors of monocyte chemotactic protein-3, CCR1 (p = 0.0001) as well as for monocyte chemotactic protein-3 (p = 0.025), CCR5 (p = 0.032) and hypoxia inducible factor-1alpha (p = 0.023). A positive relationship between vaginal distention duration and monocyte chemotactic protein-3 expression was also observed in rectal tissue (p = 0.035). Urethral expression of CCR2, another receptor for monocyte chemotactic protein-3, approached significance (p = 0.066). An inverse relationship between vaginal distention duration and interleukin-8 expression was found in the bladder (p = 0.0008). No association was noted between vaginal distention duration and the expression of stromal derived factor-1, CXCR4, CCR3 and vascular endothelial growth factor in any pelvic organs. CONCLUSIONS: These data support a relationship between vaginal distention duration and the subsequent expression of monocyte chemotactic protein-3 and 1 of its associated receptors, CCR1, in the urethra immediately following vaginal distention. The increase in hypoxia-inducible factor1alpha expression in the urethra with prolonged vaginal distention suggests a limited role of tissue ischemia in the immediate response of pelvic organs to vaginal distention.
Authors: H Q Pan; J M Kerns; D L Lin; S Liu; N Esparza; M S Damaser Journal: Am J Physiol Regul Integr Comp Physiol Date: 2007-01-04 Impact factor: 3.619
Authors: T Yoshimura; K Matsushima; S Tanaka; E A Robinson; E Appella; J J Oppenheim; E J Leonard Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205
Authors: Susanne Kaesler; Philippe Bugnon; Ji-Liang Gao; Philip M Murphy; Andreas Goppelt; Sabine Werner Journal: Wound Repair Regen Date: 2004 Mar-Apr Impact factor: 3.617
Authors: Arman T Askari; Samuel Unzek; Zoran B Popovic; Corey K Goldman; Farhad Forudi; Matthew Kiedrowski; Aleksandr Rovner; Stephen G Ellis; James D Thomas; Paul E DiCorleto; Eric J Topol; Marc S Penn Journal: Lancet Date: 2003-08-30 Impact factor: 79.321
Authors: J L Gao; T A Wynn; Y Chang; E J Lee; H E Broxmeyer; S Cooper; H L Tiffany; H Westphal; J Kwon-Chung; P M Murphy Journal: J Exp Med Date: 1997-06-02 Impact factor: 14.307
Authors: Vipul R Sheth; Pamela Duran; Jonathan Wong; Sameer Shah; Jiang Du; Karen L Christman; Eric Y Chang; Marianna Alperin Journal: J Anat Date: 2019-02-10 Impact factor: 2.610
Authors: Kristine Janssen; Dan Li Lin; Brett Hanzlicek; Kangli Deng; Brian M Balog; Carl H van der Vaart; Margot S Damaser Journal: Am J Physiol Renal Physiol Date: 2019-08-14
Authors: Andrew T Lenis; Mei Kuang; Lynn L Woo; Adonis Hijaz; Marc S Penn; Robert S Butler; Raymond Rackley; Margot S Damaser; Hadley M Wood Journal: J Urol Date: 2012-09-25 Impact factor: 7.450
Authors: Bruna M Couri; Andrew T Lenis; Ali Borazjani; Brian M Balog; Mei Kuang; Robert S Butler; Marc S Penn; Margot S Damaser Journal: Female Pelvic Med Reconstr Surg Date: 2017 Nov/Dec Impact factor: 2.091
Authors: Charuspong Dissaranan; Michelle A Cruz; Matthew J Kiedrowski; Brian M Balog; Bradley C Gill; Marc S Penn; Howard B Goldman; Margot S Damaser Journal: Cell Transplant Date: 2013-07-17 Impact factor: 4.064