OBJECTIVE: To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN: Animal study. SETTING: University laboratory. ANIMAL(S): CD-1 mice. INTERVENTION(S): Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium. MAIN OUTCOME MEASURE(S): Oocyte maturation, fertilization, and early embryonic development in vitro. RESULT(S): After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls. CONCLUSION(S): Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
OBJECTIVE: To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. DESIGN: Animal study. SETTING: University laboratory. ANIMAL(S): CD-1 mice. INTERVENTION(S): Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium. MAIN OUTCOME MEASURE(S): Oocyte maturation, fertilization, and early embryonic development in vitro. RESULT(S): After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls. CONCLUSION(S): Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
Authors: Ahmed M Taiyeb; Saeeda A Muhsen-Alanssari; W L Dees; Mundhir T Ridha-Albarzanchi; Duane C Kraemer Journal: Exp Biol Med (Maywood) Date: 2014-09-21
Authors: Min Li; Yang Yu; Jie Yan; Li-Ying Yan; Yue Zhao; Rong Li; Ping Liu; Aaron J Hsueh; Jie Qiao Journal: PLoS One Date: 2012-01-24 Impact factor: 3.240