Literature DB >> 18553863

Growth of animal cells on microbeads. I. In situ estimation of numbers.

S J Miller1, M Henrotte, A O Miller.   

Abstract

Evaluation of the number of anchorage-dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.

Year:  1986        PMID： 18553863     DOI： 10.1002/bit.260281004

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  5 in total

1.  Fastin situ determination of the biomass of anchorage-dependent cells.

Authors:  V Degouys; J Harfield; L Fabry; F D Menozzi; A O Miller
Journal:  Cytotechnology       Date:  1995-06       Impact factor: 2.058

2.  Microsupport with two-dimensional geometry (2D-MS). In situ determination of the growth kinetics of anchorage-dependent cells by Laser Diffraction Particle Sizing (LDPS).

Authors:  N Kenda-Ropson; D Mention; V Motte; M Genlain; A O Miller
Journal:  Cytotechnology       Date:  2001-09       Impact factor: 2.058

3.  Dielectric spectroscopy of mammalian cells. 2. Simultaneous in situ evaluation by aperture impedance pulse spectroscopy and low frequency dielectric spectroscopy of the biomass of HTC cells on Cytodex 3.

Authors:  V Degouys; I Cerckel; A Garcia; J Harfield; D Dubois; L Fabry; A O Miller
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

4.  Automated monitoring of cell concentration and viability using an image analysis system.

Authors:  F Maruhashi; S Murakami; K Baba
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

5.  Dielectric spectroscopy of mammalian cells. 1. Evaluation of the biomass of HeLa- and CHO cells in suspension by low-frequency dielectric spectroscopy.

Authors:  I Cerckel; A Garcia; V Degouys; D Dubois; L Fabry; A O Miller
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058
  5 in total

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