E-cadherin core fucosylation regulates nuclear beta-catenin accumulation in lung cancer cells.

E-cadherin expressed highly in 95C and lowly in 95D lung cancer cells which were from the same patient, but core-fucosylated E-cadherin highly expressed in 95D cells. Therefore, Fut8 and Fut8-RNAi constructs were transfected into 95C and 95D cells, respectively. In Fut8-transfectants, reduction of nuclear beta-catenin was noted when E-cadherin was core-fucosylated, while accumulation of nuclear beta-catenin was observed in Fut8-RNAi transfectants. In E-cadherin-negative MDA-MB-231 cells either Fut8 or Fut8-RNAi transfection couldn't affect nuclear beta-catenin. However, cotransfection of E-cadherin with Fut8 caused nuclear beta-catenin reduction. Furthermore, enhanced binding of E-cadheirn with beta-catenin as well as alpha-catenin were observed in Fut8-transfectants, and reduction of tyrosine 654 phosphorylation on beta-catenin and its transcriptional activity were also noted at the same time. Overall, the current results suggested that core-fucosylated E-cadherin regulated nuclear beta-catenin accumulation in lung cancer cells.