STUDY DESIGN: Studies were approved by the authors' Human Subjects Institutional Review Board and Institutional Animal Care and Use Committee. Anulus tissue was used in studies of the immunocytochemical localization of pregnancy-associated plasma protein A (PAPP-A) in disc tissue from the sand rat and from human disc surgical specimens and specimens from control donors. Cultured human disc cells were also tested for production of PAPP-A. OBJECTIVES: (1) To determine the immunohistochemical localization of PAPP-A in human and sand rat discs; (2) To test for gene expression of PAPP-A in the human disc in vivo and in vitro production by cultured cells; and (3) To test for expression of insulin-like growth factor binding proteins (IGFBP)-2, -4, and -5 in vivo and in vitro by human disc cells. SUMMARY OF BACKGROUND DATA: PAPP-A is a metalloproteinase expressed by several cell types, including fibroblasts, osteoblasts, and smooth muscle cells. PAPP-A has an extremely important role because it cleaves IGFBP-2, -4, and -5 in the extracellular matrix, thereby increasing the bioavailability of IGF to nearby cells. METHODS.: Specimens of human disc tissue and lumbar discs from sand rats were assessed for immunocytochemical localization of PAPP-A, and the percentage of positive cells determined. Human disc cells in three-dimensional culture were assessed for production of PAPP-A using an enzyme linked immunosorbent assay. Molecular gene expression studies were carried out using microarray analysis. RESULTS: Positive cytoplasmic immunolocalization of PAPP-A was present in the majority of cells of the human and sand rat outer anulus (OA). In the human outer anulus, the percentage of cells positive for PAPP-A localization did not differ in Grades I-II discs vs. Grades III-V discs (OA: 77.4% +/- 10.5 vs. 75.1% +/- 7.4 [mean +/- SEM] respectively). In the inner anulus, however, the percentage of cells positive for PAPP-A localization in more degenerate discs was significantly greater than the percentage in healthier discs (60.7% +/- 10.1 vs. 15.6 +/- 5.4, P = 0.024). % positive cells in the inner anulus correlated significantly with disc grade (r = 0.579; P = 0.01). Over a 5-day three-dimensional culture period, human anulus cells produced and secreted abundant PAPP-A into the culture media. Molecular studies confirmed the expression of IGFBP-2, -4, and -5 both in vivo and in vitro. CONCLUSIONS: Data provide important new insights into disc cell expression of PAPP-A at the translational level. The presence of a significantly greater proportion of cells positive for PAPP-A in the inner anulus of more degenerate Grade III-V discs compared with healthier Grade I-II discs supports our previous observation of increased gene expression of PAPP-A in more degenerated discs. Biochemical data shown here documented production of PAPP-A by disc cells in vitro. Production of PAPP-A by disc cells is important since PAPP-A cleaves IGF-binding proteins, and makes IGF-I, a potent mitogen and antiapoptotic agent, available to cells. Future studies are indicated to further investigate PAPP-A and IGF-BP function in the disc.
STUDY DESIGN: Studies were approved by the authors' Human Subjects Institutional Review Board and Institutional Animal Care and Use Committee. Anulus tissue was used in studies of the immunocytochemical localization of pregnancy-associated plasma protein A (PAPP-A) in disc tissue from the sand rat and from human disc surgical specimens and specimens from control donors. Cultured human disc cells were also tested for production of PAPP-A. OBJECTIVES: (1) To determine the immunohistochemical localization of PAPP-A in human and sand rat discs; (2) To test for gene expression of PAPP-A in the human disc in vivo and in vitro production by cultured cells; and (3) To test for expression of insulin-like growth factor binding proteins (IGFBP)-2, -4, and -5 in vivo and in vitro by human disc cells. SUMMARY OF BACKGROUND DATA: PAPP-A is a metalloproteinase expressed by several cell types, including fibroblasts, osteoblasts, and smooth muscle cells. PAPP-A has an extremely important role because it cleaves IGFBP-2, -4, and -5 in the extracellular matrix, thereby increasing the bioavailability of IGF to nearby cells. METHODS.: Specimens of human disc tissue and lumbar discs from sand rats were assessed for immunocytochemical localization of PAPP-A, and the percentage of positive cells determined. Human disc cells in three-dimensional culture were assessed for production of PAPP-A using an enzyme linked immunosorbent assay. Molecular gene expression studies were carried out using microarray analysis. RESULTS: Positive cytoplasmic immunolocalization of PAPP-A was present in the majority of cells of the human and sand rat outer anulus (OA). In the human outer anulus, the percentage of cells positive for PAPP-A localization did not differ in Grades I-II discs vs. Grades III-V discs (OA: 77.4% +/- 10.5 vs. 75.1% +/- 7.4 [mean +/- SEM] respectively). In the inner anulus, however, the percentage of cells positive for PAPP-A localization in more degenerate discs was significantly greater than the percentage in healthier discs (60.7% +/- 10.1 vs. 15.6 +/- 5.4, P = 0.024). % positive cells in the inner anulus correlated significantly with disc grade (r = 0.579; P = 0.01). Over a 5-day three-dimensional culture period, human anulus cells produced and secreted abundant PAPP-A into the culture media. Molecular studies confirmed the expression of IGFBP-2, -4, and -5 both in vivo and in vitro. CONCLUSIONS: Data provide important new insights into disc cell expression of PAPP-A at the translational level. The presence of a significantly greater proportion of cells positive for PAPP-A in the inner anulus of more degenerate Grade III-V discs compared with healthier Grade I-II discs supports our previous observation of increased gene expression of PAPP-A in more degenerated discs. Biochemical data shown here documented production of PAPP-A by disc cells in vitro. Production of PAPP-A by disc cells is important since PAPP-A cleaves IGF-binding proteins, and makes IGF-I, a potent mitogen and antiapoptotic agent, available to cells. Future studies are indicated to further investigate PAPP-A and IGF-BP function in the disc.