AIMS: To assess fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor-B (VEGF-B) effects on flow reserve and morphological adaptation in the rabbit ischemic hind limb. METHODS: Following bilateral femoral artery ligation, calf blood pressure (C(BP)), flow reserve, collateral artery numbers and capillary numbers were assessed. Treatment consisted of rabbit serum albumin (RSA), FGF-2, VEGF-B or FGF-2 + VEGF-B. RESULTS: Ligation decreased C(BP); on day 14, a 48% deficit remained in the RSA group compared with a deficit of only 22% in FGF-2 and VEGF-B groups. On day 3, flow reserve was attenuated 60%, but recovered by day 14 (with no treatment effects). Collateral artery numbers increased with RSA (+28%), FGF-2 (+53%), VEGF-B (+47%) and FGF-2 + VEGF-B (+59%). Rectus femoris muscle total capillary profiles and fibers per cross-section were alike across groups. Tibialis anterior muscle cross-sectional area was lower with ligation and total capillary number was less in RSA and FGF-2 groups, providing evidence for angiogenesis with VEGF-B. Capillary/muscle fiber ratio was similar in each group. CONCLUSIONS: FGF-2 and VEGF-B enhanced lower limb perfusion as indicated by improved C(BP) and combined treatment increased collateral artery number. Flow reserve recovery was not enhanced by cytokine treatment. VEGF-B, but not FGF-2, caused angiogenesis in the tibialis anterior muscle. Overall, VEGF-B may have advantages over FGF-2 in this setting; however, their combination may further improve arteriogenesis. Copyright 2008 S. Karger AG, Basel.
AIMS: To assess fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor-B (VEGF-B) effects on flow reserve and morphological adaptation in the rabbit ischemic hind limb. METHODS: Following bilateral femoral artery ligation, calf blood pressure (C(BP)), flow reserve, collateral artery numbers and capillary numbers were assessed. Treatment consisted of rabbit serum albumin (RSA), FGF-2, VEGF-B or FGF-2 + VEGF-B. RESULTS: Ligation decreased C(BP); on day 14, a 48% deficit remained in the RSA group compared with a deficit of only 22% in FGF-2 and VEGF-B groups. On day 3, flow reserve was attenuated 60%, but recovered by day 14 (with no treatment effects). Collateral artery numbers increased with RSA (+28%), FGF-2 (+53%), VEGF-B (+47%) and FGF-2 + VEGF-B (+59%). Rectus femoris muscle total capillary profiles and fibers per cross-section were alike across groups. Tibialis anterior muscle cross-sectional area was lower with ligation and total capillary number was less in RSA and FGF-2 groups, providing evidence for angiogenesis with VEGF-B. Capillary/muscle fiber ratio was similar in each group. CONCLUSIONS:FGF-2 and VEGF-B enhanced lower limb perfusion as indicated by improved C(BP) and combined treatment increased collateral artery number. Flow reserve recovery was not enhanced by cytokine treatment. VEGF-B, but not FGF-2, caused angiogenesis in the tibialis anterior muscle. Overall, VEGF-B may have advantages over FGF-2 in this setting; however, their combination may further improve arteriogenesis. Copyright 2008 S. Karger AG, Basel.
Authors: Fan Zhang; Zhongshu Tang; Xu Hou; Johan Lennartsson; Yang Li; Alexander W Koch; Pierre Scotney; Chunsik Lee; Pachiappan Arjunan; Lijin Dong; Anil Kumar; Tuomas T Rissanen; Bin Wang; Nobuo Nagai; Pierre Fons; Robert Fariss; Yongqing Zhang; Eric Wawrousek; Ginger Tansey; James Raber; Guo-Hua Fong; Hao Ding; David A Greenberg; Kevin G Becker; Jean-Marc Herbert; Andrew Nash; Seppo Yla-Herttuala; Yihai Cao; Ryan J Watts; Xuri Li Journal: Proc Natl Acad Sci U S A Date: 2009-04-06 Impact factor: 11.205
Authors: Cansu Yıldırım; Daphne Y S Vogel; Maurits R Hollander; Josefien M Baggen; Ruud D Fontijn; Sylvia Nieuwenhuis; Anouk Haverkamp; Margreet R de Vries; Paul H A Quax; Juan J Garcia-Vallejo; Anja M van der Laan; Christine D Dijkstra; Tineke C T M van der Pouw Kraan; Niels van Royen; Anton J G Horrevoets Journal: PLoS One Date: 2015-04-17 Impact factor: 3.240
Authors: Joana Mesquita; João Paulo Castro de Sousa; Sara Vaz-Pereira; Arminda Neves; Paulo Tavares-Ratado; Fátima M Santos; Luís A Passarinha; Cândida T Tomaz Journal: Med Sci (Basel) Date: 2017-08-09