Literature DB >> 18550297

Marker removal from actinomycetes genome using Flp recombinase.

Marta Fedoryshyn1, Lutz Petzke, Elisabeth Welle, Andreas Bechthold, Andriy Luzhetskyy.   

Abstract

We report here a system for the functional expression of the Flp recombinase in several actinomycetes: Streptomyces coelicolor, S. lividans, and Saccharotrix espanaensis. We have constructed a synthetic gene encoding the Flp recombinase with a GC content of 60.6% optimized for expression in high-GC bacteria. Using the synthetic flp(a) gene, we have removed an apramycin resistance gene flanked by FRT sites from the chromosome of actinomycetes with an efficiency of 40%. Sequencing the region of chromosome showed that excision of the apramycin cassette by Flp recombinase was specific.

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Year:  2008        PMID： 18550297     DOI： 10.1016/j.gene.2008.04.011

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  16 in total

1.  Modular and Integrative Vectors for Synthetic Biology Applications in Streptomyces spp.

Authors:  Céline Aubry; Jean-Luc Pernodet; Sylvie Lautru
Journal:  Appl Environ Microbiol       Date:  2019-08-01       Impact factor: 4.792

2.  Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.

Authors:  M Lopatniuk; B Ostash; R Makitrynskyy; S Walker; A Luzhetskyy; V Fedorenko
Journal:  J Appl Genet       Date:  2015-03-24       Impact factor: 3.240

3.  Site-specific recombination strategies for engineering actinomycete genomes.

Authors:  Simone Herrmann; Theresa Siegl; Marta Luzhetska; Lutz Petzke; Caroline Jilg; Elisabeth Welle; Annette Erb; Peter F Leadlay; Andreas Bechthold; Andriy Luzhetskyy
Journal:  Appl Environ Microbiol       Date:  2012-01-13       Impact factor: 4.792

4.  Formation and attachment of the deoxysugar moiety and assembly of the gene cluster for caprazamycin biosynthesis.

Authors:  Leonard Kaysser; Emmanuel Wemakor; Stefanie Siebenberg; Jose A Salas; Jae Kyung Sohng; Bernd Kammerer; Bertolt Gust
Journal:  Appl Environ Microbiol       Date:  2010-04-23       Impact factor: 4.792

5.  Identification of a gene from Streptomyces rimosus M527 negatively affecting rimocidin biosynthesis and morphological differentiation.

Authors:  Zhijun Liao; Zhangqing Song; Jie Xu; Zheng Ma; Andreas Bechthold; Xiaoping Yu
Journal:  Appl Microbiol Biotechnol       Date:  2020-10-15       Impact factor: 4.813

Review 6.  Engineering microbial hosts for production of bacterial natural products.

Authors:  Mingzi M Zhang; Yajie Wang; Ee Lui Ang; Huimin Zhao
Journal:  Nat Prod Rep       Date:  2016-04-13       Impact factor: 13.423

7.  Pleiotropic role of the Sco1/SenC family copper chaperone in the physiology of Streptomyces.

Authors:  Masahiro Fujimoto; Akio Yamada; Junpei Kurosawa; Akihiro Kawata; Teruhiko Beppu; Hideaki Takano; Kenji Ueda
Journal:  Microb Biotechnol       Date:  2011-11-24       Impact factor: 5.813

8.  An efficient procedure for marker-free mutagenesis of S. coelicolor by site-specific recombination for secondary metabolite overproduction.

Authors:  Bo Zhang; Lin Zhang; Ruixue Dai; Meiying Yu; Guoping Zhao; Xiaoming Ding
Journal:  PLoS One       Date:  2013-02-07       Impact factor: 3.240

9.  A novel method to generate unmarked gene deletions in the intracellular pathogen Rhodococcus equi using 5-fluorocytosine conditional lethality.

Authors:  R van der Geize; W de Jong; G I Hessels; A W F Grommen; A A C Jacobs; L Dijkhuizen
Journal:  Nucleic Acids Res       Date:  2008-11-04       Impact factor: 16.971

10.  Heterologous expression of the thiopeptide antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor requires deletion of ribosomal genes from the expression construct.

Authors:  Katrin Flinspach; Claudia Kapitzke; Arianna Tocchetti; Margherita Sosio; Alexander K Apel
Journal:  PLoS One       Date:  2014-03-05       Impact factor: 3.240
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