Literature DB >> 1854600

Detection of Clostridium perfringens type A enterotoxin in faecal and food samples using immunomagnetic separation (IMS)-ELISA.

K S Cudjoe1, L I Thorsen, T Sørensen, J Reseland, O Olsvik, P E Granum.   

Abstract

A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.

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Year:  1991        PMID: 1854600     DOI: 10.1016/0168-1605(91)90145-f

Source DB:  PubMed          Journal:  Int J Food Microbiol        ISSN: 0168-1605            Impact factor:   5.277


  8 in total

Review 1.  Clostridial enteric diseases of domestic animals.

Authors:  J G Songer
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

2.  Rapid detection of Shigella dysenteriae and Shigella flexneri in faeces by an immunomagnetic assay with monoclonal antibodies.

Authors:  D Islam; S Tzipori; M Islam; A A Lindberg
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-01       Impact factor: 3.267

3.  Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.

Authors:  L A Baez; V K Juneja
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

4.  Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction.

Authors:  D Islam; A A Lindberg
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

5.  Immunomagnetic PCR and DNA probe for detection and identification of Porphyromonas gingivalis.

Authors:  R M Benkirane; E Guillot; C Mouton
Journal:  J Clin Microbiol       Date:  1995-11       Impact factor: 5.948

Review 6.  Magnetic separation techniques in diagnostic microbiology.

Authors:  O Olsvik; T Popovic; E Skjerve; K S Cudjoe; E Hornes; J Ugelstad; M Uhlén
Journal:  Clin Microbiol Rev       Date:  1994-01       Impact factor: 26.132

7.  A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles.

Authors:  Zhiqiang Shen; Nannan Hou; Min Jin; Zhigang Qiu; Jingfeng Wang; Bin Zhang; Xinwei Wang; Jie Wang; Dongsheng Zhou; Junwen Li
Journal:  Gut Pathog       Date:  2014-05-20       Impact factor: 4.181

8.  Colorimetric detection of Shewanella oneidensis based on immunomagnetic capture and bacterial intrinsic peroxidase activity.

Authors:  Junlin Wen; Shungui Zhou; Junhua Chen
Journal:  Sci Rep       Date:  2014-06-05       Impact factor: 4.379

  8 in total

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