| Literature DB >> 1854600 |
K S Cudjoe1, L I Thorsen, T Sørensen, J Reseland, O Olsvik, P E Granum.
Abstract
A simple, rapid and sensitive immunoassay, based on immunomagnetic particles (Dynabeads M-280) was developed for detection and quantitation of Clostridium perfringens type A enterotoxin from faecal and food extracts. The assay had a detection limit of 2.5 ng/ml enterotoxin in homogenates of faeces and inoculated meat extracts. The specificity was confirmed by both crossed immunoelectrophoresis and Western immunoblotting techniques, using a purified enterotoxin as standard.Entities:
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Year: 1991 PMID: 1854600 DOI: 10.1016/0168-1605(91)90145-f
Source DB: PubMed Journal: Int J Food Microbiol ISSN: 0168-1605 Impact factor: 5.277