Literature DB >> 18545580

Simple and robust image-based autofocusing for digital microscopy.

Siavash Yazdanfar1, Kevin B Kenny, Krenar Tasimi, Alex D Corwin, Elizabeth L Dixon, Robert J Filkins.   

Abstract

A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.

Mesh:

Year:  2008        PMID: 18545580     DOI: 10.1364/oe.16.008670

Source DB:  PubMed          Journal:  Opt Express        ISSN: 1094-4087            Impact factor:   3.894


  20 in total

1.  InstantScope: a low-cost whole slide imaging system with instant focal plane detection.

Authors:  Kaikai Guo; Jun Liao; Zichao Bian; Xin Heng; Guoan Zheng
Journal:  Biomed Opt Express       Date:  2015-08-04       Impact factor: 3.732

2.  Compact multi-band fluorescent microscope with an electrically tunable lens for autofocusing.

Authors:  Zhaojun Wang; Ming Lei; Baoli Yao; Yanan Cai; Yansheng Liang; Yanlong Yang; Xibin Yang; Hui Li; Daxi Xiong
Journal:  Biomed Opt Express       Date:  2015-10-14       Impact factor: 3.732

3.  Autofocus method for automated microscopy using embedded GPUs.

Authors:  J M Castillo-Secilla; M Saval-Calvo; L Medina-Valdès; S Cuenca-Asensi; A Martínez-Álvarez; C Sánchez; G Cristóbal
Journal:  Biomed Opt Express       Date:  2017-02-22       Impact factor: 3.732

4.  Single-frame rapid autofocusing for brightfield and fluorescence whole slide imaging.

Authors:  Jun Liao; Liheng Bian; Zichao Bian; Zibang Zhang; Charmi Patel; Kazunori Hoshino; Yonina C Eldar; Guoan Zheng
Journal:  Biomed Opt Express       Date:  2016-10-27       Impact factor: 3.732

5.  Transform- and multi-domain deep learning for single-frame rapid autofocusing in whole slide imaging.

Authors:  Shaowei Jiang; Jun Liao; Zichao Bian; Kaikai Guo; Yongbing Zhang; Guoan Zheng
Journal:  Biomed Opt Express       Date:  2018-03-08       Impact factor: 3.732

6.  All-optical microscope autofocus based on an electrically tunable lens and a totally internally reflected IR laser.

Authors:  M Bathe-Peters; P Annibale; M J Lohse
Journal:  Opt Express       Date:  2018-02-05       Impact factor: 3.894

7.  Automated focusing in bright-field microscopy for tuberculosis detection.

Authors:  O A Osibote; R Dendere; S Krishnan; T S Douglas
Journal:  J Microsc       Date:  2010-11       Impact factor: 1.758

Review 8.  Using time-lapse fluorescence microscopy to study gene regulation.

Authors:  Fan Zou; Lu Bai
Journal:  Methods       Date:  2018-12-29       Impact factor: 3.608

9.  Rapid and robust whole slide imaging based on LED-array illumination and color-multiplexed single-shot autofocusing.

Authors:  Shaowei Jiang; Zichao Bian; Xizhi Huang; Pengming Song; He Zhang; Yongbing Zhang; Guoan Zheng
Journal:  Quant Imaging Med Surg       Date:  2019-05

10.  Focus on time: dynamic imaging reveals stretch-dependent cell relaxation and nuclear deformation.

Authors:  Aron N Horvath; Andreas A Ziegler; Stephan Gerhard; Claude N Holenstein; Benjamin Beyeler; Jess G Snedeker; Unai Silvan
Journal:  Biophys J       Date:  2021-01-30       Impact factor: 4.033

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