Literature DB >> 1854340

Requirements for the inactivation of ribosomes by gelonin.

S Sperti1, M Brigotti, M Zamboni, D Carnicelli, L Montanaro.   

Abstract

Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP and a high-Mr factor present in the rabbit reticulocyte-lysate post-ribosomal supernatant. The kinetic constants of the gelonin-catalysed release of adenine from A. salina ribosomes are Km = 4.35 microM and Kcat. = 0.1 min-1 in the absence of cofactors, and Km = 1.15 microM and Kcat. = 108 min-1 in their presence. The last two values are similar to those measured for ricin A chain in the absence of cofactors (Km = 2.02 microM and Kcat. = 317 min-1).

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Year:  1991        PMID： 1854340      PMCID： PMC1151221          DOI： 10.1042/bj2770281

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

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3.  Dye affinity chromatography of ricin subunits.

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5.  The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins.

Authors:  Y Endo; K Tsurugi; J M Lambert
Journal:  Biochem Biophys Res Commun       Date:  1988-02-15       Impact factor: 3.575

6.  Modification of ribosomal RNA by ribosome-inactivating proteins from plants.

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Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

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8.  Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA.

Authors:  D Moazed; J M Robertson; H F Noller
Journal:  Nature       Date:  1988-07-28       Impact factor: 49.962

9.  High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin.

Authors:  M Zamboni; M Brigotti; F Rambelli; L Montanaro; S Sperti
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

10.  Effect of alpha-sarcin and ribosome-inactivating proteins on the interaction of elongation factors with ribosomes.

Authors:  M Brigotti; F Rambelli; M Zamboni; L Montanaro; S Sperti
Journal:  Biochem J       Date:  1989-02-01       Impact factor: 3.857
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  9 in total

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Authors:  Maurizio Brigotti; Pier Giorgio Petronini; Domenica Carnicelli; Roberta R Alfieri; Mara A Bonelli; Angelo F Borghetti; Kenneth P Wheeler
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2.  Biodistribution, pharmacokinetics, and nuclear imaging studies of 111In-labeled rGel/BLyS fusion toxin in SCID mice bearing B cell lymphoma.

Authors:  Xiaoxia Wen; Mi-Ae Lyu; Rui Zhang; Wei Lu; Qian Huang; Dong Liang; Michael G Rosenblum; Chun Li
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3.  3'-immature tRNA(Trp) is required for ribosome inactivation by gelonin,a plant RNA N-glycosidase.

Authors:  M Brigotti; D Carnicelli; P Alvergna; A Pallanca; R Lorenzetti; M Denaro; S Sperti; L Montanaro
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

4.  Effects of ribosome-inactivating proteins on Escherichia coli and Agrobacterium tumefaciens translation systems.

Authors:  T Girbés; L Barbieri; M Ferreras; F J Arias; M A Rojo; R Iglesias; C Alegre; C Escarmis; F Stirpe
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490

5.  Wheat ribosome-inactivating proteins: seed and leaf forms with different specificities and cofactor requirements.

Authors:  A J Massiah; M R Hartley
Journal:  Planta       Date:  1995       Impact factor: 4.116

6.  Maize ribosome-inactivating protein (b-32). Homologs in related species, effects on maize ribosomes, and modulation of activity by pro-peptide deletions.

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7.  In vitro and in vivo studies of a VEGF121/rGelonin chimeric fusion toxin targeting the neovasculature of solid tumors.

Authors:  Liesbeth M Veenendaal; Hangqing Jin; Sophia Ran; Lawrence Cheung; Nora Navone; John W Marks; Johannes Waltenberger; Philip Thorpe; Michael G Rosenblum
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

8.  A reconstituted cell-free assay for the evaluation of the intrinsic activity of purified human ribosomes.

Authors:  Marianna Penzo; Domenica Carnicelli; Lorenzo Montanaro; Maurizio Brigotti
Journal:  Nat Protoc       Date:  2016-06-23       Impact factor: 13.491

Review 9.  Functional Assays for Measuring the Catalytic Activity of Ribosome Inactivating Proteins.

Authors:  Yijun Zhou; Xiao-Ping Li; Jennifer N Kahn; Nilgun E Tumer
Journal:  Toxins (Basel)       Date:  2018-06-14       Impact factor: 4.546
  9 in total

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