| Literature DB >> 18542886 |
Erkang Fan1, David Baker, Stanley Fields, Michael H Gelb, Frederick S Buckner, Wesley C Van Voorhis, Eric Phizicky, Mark Dumont, Christopher Mehlin, Elizabeth Grayhack, Mark Sullivan, Christophe Verlinde, George Detitta, Deirdre R Meldrum, Ethan A Merritt, Thomas Earnest, Michael Soltis, Frank Zucker, Peter J Myler, Lori Schoenfeld, David Kim, Liz Worthey, Doug Lacount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna Massey, Brian Carroll, Stacey Gulde, Joseph Luft, Larry Desoto, Mark Holl, Jonathan Caruthers, Jürgen Bosch, Mark Robien, Tracy Arakaki, Margaret Holmes, Isolde Le Trong, Wim G J Hol.
Abstract
The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.Entities:
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Year: 2008 PMID： 18542886 DOI： 10.1007/978-1-60327-058-8_33
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745