Automatic microarray spot segmentation using a Snake-Fisher model.

Inspired by Paragious and Deriche's work, which unifies boundary-based and region-based image partition approaches, we integrate the snake model and the Fisher criterion to capture, respectively, the boundary information and region information of microarray images. We then use the proposed algorithm to segment the spots in the microarray images, and compare our results with those obtained by commercial software. Our algorithm is automatic because the parameters are adaptively estimated from the data without human intervention.