| Literature DB >> 18539482 |
Tang Wang1, Zhizhen Qi, Benchuan Wu, Ziwen Zhu, Yonghai Yang, Baizhong Cui, Ruixia Dai, Qingwen Zhang, Yefeng Qiu, Zuyun Wang, Hu Wang, Zhaobiao Guo, Xiaoyi Wang, Ruifu Yang.
Abstract
F1 antigen is an attractive candidate for the development of a subunit vaccine against plague. In previous study, the extraction of this antigen from Yersinia pestis is characterized by using organic solvents. In this work, a new purification strategy that produced high-purity F1 antigen from Y. pestis EV76 was developed by the substitution of physical disruption for organic solvent one, followed by a combination of ammonium sulfate fractionation and Sephacryl S-200HR column filtration chromatography. As revealed in this study, this purification procedure is simple and effective, and avoids potential adverse effect on the antigen by organic solvents. Highly purified F1 that adsorbed to 25% (v/v) Al(OH)3 adjuvant in phosphate-buffered saline (PBS) induced very high titers of antibody to F1 in BALB/c mice and protected them (100% survival) against subcutaneous challenge with 10(4) CFU of Y. pestis virulent strain 141.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18539482 DOI： 10.1016/j.pep.2008.05.003
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650