Interspecific variation in bulk tissue, fatty acid and monosaccharide delta(13)C values of leaves from a mesotrophic grassland plant community.

The leaves of 37 grass, herb, shrub and tree species were collected from a mesotrophic grassland to assess natural variability in bulk, fatty acid and monosaccharide delta(13)C values of leaves from one plant community. The leaf tissue mean bulk delta(13)C value was -29.3 per thousand. No significant differences between tissue bulk delta(13)C values with life form were determined (P=0.40). On average, C(16:0), C(18:2) and C(18:3) constituted 89% of leaf tissue total fatty acids, whose delta(13)C values were depleted compared to whole leaf tissues. A general interspecific (between different species) trend for fatty acids delta(13)C values was observed, i.e. delta(13)C(16:0)<delta(13)C(18:2)<delta(13)C(18:3), although these values ranged widely between species, e.g. C(16:0) (-34.7 per thousand, Alisma plantago-aquatica; -44.0 per thousand, Leucanthemum vulgare), C(18:2) (-33.3 per thousand, Acer campestre; -44.2 per thousand, L. vulgare;) and C(18:3) (-34.3 per thousand, Bellis perennis; -41.8 per thousand, Plantago lanceolata). Average relative abundances of leaf monosaccharides arabinose, xylose, mannose, galactose and glucose were 12%, 13%, 5%, 12% and 54%, respectively. Mean delta(13)C values of these monosaccharides were -26.6 per thousand (arabinose), -27.2 per thousand (xylose), -30.9 per thousand (mannose), -30.0 per thousand (galactose) and -29.0 per thousand (glucose). The general relationship between individual monosaccharide delta(13)C values, delta(13)C(arabinose)>delta(13)C(xylose)>delta(13)C(glucose)>delta(13)C(galactose), was consistently observed. Therefore, we have shown (i) diversity in compound-specific delta(13)C values contributing to leaf bulk delta(13)C values; (ii) interspecific variability between bulk and compound-specific delta(13)C values of leaves of individual grassland species, and (iii) trends between individual fatty acid and monosaccharide delta(13)C values common to leaves of all species within one plant community.