PURPOSE: We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. PROCEDURES: Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. RESULTS: Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. CONCLUSION: PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries.
PURPOSE: We investigated in vitro the potential of macrophages to act as targeted vehicle for ultrasound molecular imaging. PROCEDURES: Murine bone marrow-derived macrophages (BMM), incubated for 3 h with different concentrations of perfluorohexane (PFH) emulsions, were monitored by microscopy, flow cytometry, and ultrasound. Effects of PFH loading on BMM adhesion molecule (PSGL-1, VLA-4, Mac-1, LFA-1) expression were analyzed by flow cytometry. Static adhesion of PFH loaded BMM to unstimulated and TNF-alpha stimulated b.End5 endothelial cells was assessed by microscopy. RESULTS: Incubation of BMM with PFH emulsions resulted in dose-dependent uptake and increased echogenicity (max. 17 dB). Flow cytometry analyses revealed no down-regulation related to PFH loading of BMM adhesion molecule expression. Endothelial adhesion remained functional, even after 24 h, although PFH loading dose-dependently attenuated static adhesion. CONCLUSION:PFH loaded BMM may potentially serve as ultrasound contrast agent for noninvasive detection of atherogenic hotspots in arteries.
Authors: R E Gerszten; Y C Lim; H T Ding; K Snapp; G Kansas; D A Dichek; C Cabañas; F Sánchez-Madrid; M A Gimbrone; A Rosenzweig; F W Luscinskas Journal: Circ Res Date: 1998-05-04 Impact factor: 17.367
Authors: G M Lanza; K D Wallace; M J Scott; W P Cacheris; D R Abendschein; D H Christy; A M Sharkey; J G Miller; P J Gaffney; S A Wickline Journal: Circulation Date: 1996-12-15 Impact factor: 29.690
Authors: H Alkan-Onyuksel; S M Demos; G M Lanza; M J Vonesh; M E Klegerman; B J Kane; J Kuszak; D D McPherson Journal: J Pharm Sci Date: 1996-05 Impact factor: 3.534
Authors: Liselotte M Kornmann; Alma Zernecke; Daniëlle M J Curfs; Ben J A Janssen; Christian Weber; Menno P J de Winther; Robert S Reneman; Arnold P G Hoeks; Koen D Reesink Journal: Cardiovasc Ultrasound Date: 2015-01-08 Impact factor: 2.062