OBJECTIVE: To study the chemical constituents in the stems of Schisandra propinqua and evaluate their effects on proliferation of vascular smooth muscle cells (VSMCs) in vitro by MTT assay. METHOD: The compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (ESI-MS, 1H-NMR, 13C-NMR). RESULT: Five compounds were isolated and identified as galgravin (1), veraguensin (2), octadecanoic acid 2, 3-dihydroxypropyl ester (3), hexadecanoic acid 2, 3-dihydroxypropy ester (4), tetracosanoic acid 2, 3-dihydroxypropyl ester (5). CONCLUSION: All the compounds were isolated for the first time from the plant. 1, 2, 5 showed inhibiting effects on vascular smooth muscle cell (VSMCs) proliferation in vitro.
OBJECTIVE: To study the chemical constituents in the stems of Schisandra propinqua and evaluate their effects on proliferation of vascular smooth muscle cells (VSMCs) in vitro by MTT assay. METHOD: The compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (ESI-MS, 1H-NMR, 13C-NMR). RESULT: Five compounds were isolated and identified as galgravin (1), veraguensin (2), octadecanoic acid 2, 3-dihydroxypropyl ester (3), hexadecanoic acid 2, 3-dihydroxypropy ester (4), tetracosanoic acid 2, 3-dihydroxypropyl ester (5). CONCLUSION: All the compounds were isolated for the first time from the plant. 1, 2, 5 showed inhibiting effects on vascular smooth muscle cell (VSMCs) proliferation in vitro.