BACKGROUND/AIM: Diabetes leads to chronic renal hypoxia and cellular hypoxia response (mediated by hypoxia-inducible factors) and predisposes to acute kidney injury. We studied the impact of acute and chronic hypoxic stress on the development of acute kidney injury in the diabetic rat kidney. METHODS: Control (CTR) and streptozotocin (STZ)-diabetic rats were studied following acute medullary hypoxic stress, induced by combinations of radiocontrast and inhibitors of cyclooxygenase and NO synthase. In addition, STZ and CTR kidneys were compared following ex vivo perfusion with oxygenated cell-free medium. RESULTS: The extents of medullary acute tubular injury and renal dysfunction were largely comparable in CTR and STZ-diabetic kidneys in vivo. By contrast, functional deterioration and outer medullary injury were markedly enhanced in STZ kidneys perfused ex vivo. A peculiar collecting duct injury pattern, with cell swelling and detachment, noted in intact STZ kidneys, prominently intensified following isolated perfusion. CONCLUSIONS: The diabetic kidney is remarkably resistant to acute hypoxic injury in vivo, possibly due to chronic hypoxia adaptation. Thus, though diabetes predisposes to acute kidney injury in various clinical settings, reduced kidney function does not necessarily imply a greater extent of true tubular damage. The collecting duct injury pattern is an as yet unrecognized feature of early experimental diabetes. Copyright 2008 S. Karger AG, Basel.
BACKGROUND/AIM: Diabetes leads to chronic renal hypoxia and cellular hypoxia response (mediated by hypoxia-inducible factors) and predisposes to acute kidney injury. We studied the impact of acute and chronic hypoxic stress on the development of acute kidney injury in the diabeticrat kidney. METHODS: Control (CTR) and streptozotocin (STZ)-diabeticrats were studied following acute medullary hypoxic stress, induced by combinations of radiocontrast and inhibitors of cyclooxygenase and NO synthase. In addition, STZ and CTR kidneys were compared following ex vivo perfusion with oxygenated cell-free medium. RESULTS: The extents of medullary acute tubular injury and renal dysfunction were largely comparable in CTR and STZ-diabetic kidneys in vivo. By contrast, functional deterioration and outer medullary injury were markedly enhanced in STZ kidneys perfused ex vivo. A peculiar collecting duct injury pattern, with cell swelling and detachment, noted in intact STZ kidneys, prominently intensified following isolated perfusion. CONCLUSIONS: The diabetic kidney is remarkably resistant to acute hypoxic injury in vivo, possibly due to chronic hypoxia adaptation. Thus, though diabetes predisposes to acute kidney injury in various clinical settings, reduced kidney function does not necessarily imply a greater extent of true tubular damage. The collecting duct injury pattern is an as yet unrecognized feature of early experimental diabetes. Copyright 2008 S. Karger AG, Basel.
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Authors: Kai-Uwe Eckardt; Philipp Enghard; Nikolaus Rajewsky; Kai M Schmidt-Ott; Christian Hinze; Christine Kocks; Janna Leiz; Nikos Karaiskos; Anastasiya Boltengagen; Shuang Cao; Christopher Mark Skopnik; Jan Klocke; Jan-Hendrik Hardenberg; Helena Stockmann; Inka Gotthardt; Benedikt Obermayer; Laleh Haghverdi; Emanuel Wyler; Markus Landthaler; Sebastian Bachmann; Andreas C Hocke; Victor Corman; Jonas Busch; Wolfgang Schneider; Nina Himmerkus; Markus Bleich Journal: Genome Med Date: 2022-09-09 Impact factor: 15.266