| Literature DB >> 18535362 |
S Rezaei-Zarchi1, A A Saboury, A Javed, A Barzegar, S Ahmadian, A Bayandori-Moghaddam.
Abstract
A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of -562 +/- 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 +/- 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 (micro)M.Entities:
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Year: 2008 PMID： 18535362 DOI： 10.1007/s12038-008-0045-4
Source DB: PubMed Journal: J Biosci ISSN： 0250-5991 Impact factor: 1.826