Literature DB >> 18528549

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.

Takakazu Nakabayashi1, Hui-Ping Wang, Masataka Kinjo, Nobuhiro Ohta.   

Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

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Year:  2008        PMID： 18528549     DOI： 10.1039/b800391b

Source DB:  PubMed          Journal:  Photochem Photobiol Sci        ISSN： 1474-905X            Impact factor:   3.982


  18 in total

Review 1.  Fluorescence lifetime measurements and biological imaging.

Authors:  Mikhail Y Berezin; Samuel Achilefu
Journal:  Chem Rev       Date:  2010-05-12       Impact factor: 60.622

2.  Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry.

Authors:  Ali Vaziri Gohar; Ruofan Cao; Patrick Jenkins; Wenyan Li; Jessica P Houston; Kevin D Houston
Journal:  Biomed Opt Express       Date:  2013-07-19       Impact factor: 3.732

3.  Sustained accurate recording of intracellular acidification in living tissues with a photo-controllable bioluminescent protein.

Authors:  Mitsuru Hattori; Sanae Haga; Hideo Takakura; Michitaka Ozaki; Takeaki Ozawa
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-20       Impact factor: 11.205

4.  Green fluorescent protein emission obscures metabolic fluorescent lifetime imaging of NAD(P)H.

Authors:  Elisa M York; Nicholas L Weilinger; Jeffrey M LeDue; Brian A MacVicar
Journal:  Biomed Opt Express       Date:  2019-08-02       Impact factor: 3.732

Review 5.  Molecular probes for fluorescence lifetime imaging.

Authors:  Pinaki Sarder; Dolonchampa Maji; Samuel Achilefu
Journal:  Bioconjug Chem       Date:  2015-05-22       Impact factor: 4.774

6.  Long fluorescence lifetime molecular probes based on near infrared pyrrolopyrrole cyanine fluorophores for in vivo imaging.

Authors:  Mikhail Y Berezin; Walter J Akers; Kevin Guo; Georg M Fischer; Ewald Daltrozzo; Andreas Zumbusch; Samuel Achilefu
Journal:  Biophys J       Date:  2009-11-04       Impact factor: 4.033

Review 7.  In Vivo Imaging with Genetically Encoded Redox Biosensors.

Authors:  Alexander I Kostyuk; Anastasiya S Panova; Aleksandra D Kokova; Daria A Kotova; Dmitry I Maltsev; Oleg V Podgorny; Vsevolod V Belousov; Dmitry S Bilan
Journal:  Int J Mol Sci       Date:  2020-10-31       Impact factor: 5.923

8.  Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breast.

Authors:  Matthew W Conklin; Paolo P Provenzano; Kevin W Eliceiri; Ruth Sullivan; Patricia J Keely
Journal:  Cell Biochem Biophys       Date:  2009       Impact factor: 2.194

9.  Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor.

Authors:  Emily P Haynes; Megha Rajendran; Chace K Henning; Abhipri Mishra; Angeline M Lyon; Mathew Tantama
Journal:  Anal Chem       Date:  2019-06-19       Impact factor: 6.986

10.  Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells.

Authors:  Kirstin Elgass; Katharina Caesar; Frank Schleifenbaum; York-Dieter Stierhof; Alfred J Meixner; Klaus Harter
Journal:  PLoS One       Date:  2009-05-27       Impact factor: 3.240
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