Use of laser fluorescence and scanning electron microscope to evaluate remineralization of incipient enamel lesions remineralized by topical application of casein phospho peptide amorphous calcium phosphate (CPP-aCP) containing cream.

The aim of this in vitro study was to evaluate the remineralization of incipient enamel lesions by the topical application of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) using laser fluorescence and scanning electron microscope. Sixty caries free extracted teeth were used in the study. Forty teeth were used as test samples, ten as positive and ten as negative controls. The samples were demineralized and then remineralized by the topical application of CPP-ACP for a period of 14 days. The remineralization was evaluated with the use of laser fluorescence and scanning electron microscope (SEM). The results of this study showed that the laser fluorescent readings of test samples after remineralization were highly significant (p < 0.001). A significant number of test samples observed under SEM showed high scores of remineralization.