BACKGROUND: Different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis B (HB) vaccine do not produce protective levels of anti-HB antibody, a phenomenon which could be linked to certain human leukocyte antigen (HLA) class-II alleles or haplotypes. OBJECTIVES: The present study was undertaken to determine the frequency of HLA class-II alleles in Iranian healthy adult responders and non-responders to HB vaccine. METHODS: Twelve non-responders (anti-HBs antibody<10 IU/L) and 46 responders (anti-HBs antibody>100 IU/L) were tissue typed for HLA class-II. HLA-DRB1, DQB1 and DQA1 alleles were determined using polymerase chain reaction based on sequence specific primers (PCR-SSP) technique. Accessibility to excess amount of genomic DNA was possible using Epstein-Barr virus (EBV)-transformed B-cells established from all vaccinees. RESULTS: Our results demonstrated increased frequencies of HLA- DRB107, DRB103, DRB104, DQB10201, DQA10201 alleles and HLA- DRB107/DQB10201/DQA10201 and DRB104/DQB10302/DQA103011 haplotypes in the non-responder group. Comparison between responders and non-responders revealed only a significant difference for DQB10201 allele (p<0.05). CONCLUSION: These findings confirm the association of certain HLA alleles and haplotypes with the lack of antibody response to HB vaccine in an Iranian population.
BACKGROUND: Different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis B (HB) vaccine do not produce protective levels of anti-HB antibody, a phenomenon which could be linked to certain human leukocyte antigen (HLA) class-II alleles or haplotypes. OBJECTIVES: The present study was undertaken to determine the frequency of HLA class-II alleles in Iranian healthy adult responders and non-responders to HB vaccine. METHODS: Twelve non-responders (anti-HBs antibody<10 IU/L) and 46 responders (anti-HBs antibody>100 IU/L) were tissue typed for HLA class-II. HLA-DRB1, DQB1 and DQA1 alleles were determined using polymerase chain reaction based on sequence specific primers (PCR-SSP) technique. Accessibility to excess amount of genomic DNA was possible using Epstein-Barr virus (EBV)-transformed B-cells established from all vaccinees. RESULTS: Our results demonstrated increased frequencies of HLA- DRB107, DRB103, DRB104, DQB10201, DQA10201 alleles and HLA- DRB107/DQB10201/DQA10201 and DRB104/DQB10302/DQA103011 haplotypes in the non-responder group. Comparison between responders and non-responders revealed only a significant difference for DQB10201 allele (p<0.05). CONCLUSION: These findings confirm the association of certain HLA alleles and haplotypes with the lack of antibody response to HB vaccine in an Iranian population.
Authors: Shu-Chen Hung; Tieying Hou; Wei Jiang; Nan Wang; Shuo-Wang Qiao; I-Ting Chow; Xiaodan Liu; Sjoerd H van der Burg; David M Koelle; William W Kwok; Ludvig M Sollid; Elizabeth D Mellins Journal: J Immunol Date: 2019-03-29 Impact factor: 5.422
Authors: Susana Hernández-Doño; Roberto Alejandro Sánchez-González; Ma Guadalupe Trujillo-Vizuet; Fabiola Yeseline Zamudio-Castellanos; Rafael García-Silva; Pedro Bulos-Rodríguez; Carlos A Vazquez-Guzmán; Ximena Cárdenas-Ramos; Diana de León Rodríguez; Fabiola Elías; Sergio Domínguez-Arrevillaga; José Manuel Pérez-Tirado; Olga Lidia Vera-Lastra; Julio Granados; Jesús Sepúlveda-Delgado Journal: Clin Immunol Date: 2022-04-05 Impact factor: 10.190