| Literature DB >> 18519040 |
Xiaoyun Wu1, You Zhou, Ke Zhang, Qingzhen Liu, Deyin Guo.
Abstract
Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.Entities:
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Year: 2008 PMID： 18519040 DOI： 10.1016/j.febslet.2008.05.033
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124