Literature DB >> 1851803

Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis.

K Omori1, S Suzuki, Y Imai, S Komatsubara.   

Abstract

The proBA operon, coding for gamma-glutamyl kinase (GK) and gamma-glutamyl phosphate reductase (GPR), was cloned from the chromosome of the wild-type strain of Serratia marcescens Sr41. From our sequence data the proB (1101 bp) and proA (1472 bp) genes were shown to code for two proteins of Mr 39,169 and 44,640, respectively. Analysis of expression of the lacZ structural gene fused with the proBA promoter showed that the proBA operon is not subject to proline-mediated feedback repression. Amplification of the proBA operon enabled us to determine GK activity, which was inhibited in the presence of a low concentration of L-proline. Comparison of the amino acid sequences of the S. marcescens GK and GPR proteins with those of the GK and GPR proteins from E. coli revealed extensive similarities.

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Year:  1991        PMID： 1851803     DOI： 10.1099/00221287-137-3-509

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  14 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-08-11       Impact factor: 16.971

2.  Nucleotide sequence of the Serratia marcescens threonine operon and analysis of the threonine operon mutations which alter feedback inhibition of both aspartokinase I and homoserine dehydrogenase I.

Authors:  K Omori; Y Imai; S Suzuki; S Komatsubara
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

3.  Corrected gene assignments of Escherichia coli pro- mutations.

Authors:  I Serebrijski; O Reyes; G Leblon
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

4.  Construction of a proline-producing mutant of the extremely thermophilic eubacterium Thermus thermophilus HB27.

Authors:  T Kosuge; T Hoshino
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

5.  Improvement of culture conditions for L-proline production by a recombinant strain of Serratia marcescens.

Authors:  M Masuda; S Takamatu; N Nishimura; S Komatsubara; T Tosa
Journal:  Appl Biochem Biotechnol       Date:  1993-12       Impact factor: 2.926

6.  Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli.

Authors:  K Yamanaka; T Ogura; E V Koonin; H Niki; S Hiraga
Journal:  Mol Gen Genet       Date:  1994-04

7.  Molecular analysis of the aspartate kinase-homoserine dehydrogenase gene from Arabidopsis thaliana.

Authors:  M Ghislain; V Frankard; D Vandenbossche; B F Matthews; M Jacobs
Journal:  Plant Mol Biol       Date:  1994-03       Impact factor: 4.076

8.  Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants.

Authors:  I Serebrijski; F Wojcik; O Reyes; G Leblon
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

9.  Role of serine 352 in the allosteric response of Serratia marcescens aspartokinase I-homoserine dehydrogenase I analyzed by using site-directed mutagenesis.

Authors:  K Omori; S Komatsubara
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490

10.  Cloning and characterization of the gamma-glutamyl phosphate reductase gene of Campylobacter jejuni.

Authors:  H Louie; V L Chan
Journal:  Mol Gen Genet       Date:  1993-07
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