OBJECTIVE: Investigation of chronic infections with Chlamydophila pneumoniae. METHODS: BALB/c mice were repeatedly infected with C. pneumoniae and tested during a 1-year period. Production of histamine, IFN-gamma, IL-6 and antibodies was monitored by ELISA. Live bacteria were cultured and DNA was detected by PCR. Cellular immunity was tested by ELISPOT. RESULTS: After re-infections, culture positivity and persistence of DNA in lungs and blood were shorter. Detection of DNA at late time points indicated persistent infection in a few mice. Histamine was produced after primary and re-infections, and the level correlated with the number of viable bacteria in lung. IFN-gamma, IL-6 levels, IgG2/IgG1 ratio, IgA titres, and level of chlamydial heat-shock protein antibodies were higher after re-infections. IgM antibodies were demonstrated even after re-infections. High number of IFN-gamma-producing splenocytes was observed after the third inoculation. CONCLUSION: These results promote an understanding of the patho- and immune mechanisms after C. pneumoniae re-infections.
OBJECTIVE: Investigation of chronic infections with Chlamydophila pneumoniae. METHODS: BALB/c mice were repeatedly infected with C. pneumoniae and tested during a 1-year period. Production of histamine, IFN-gamma, IL-6 and antibodies was monitored by ELISA. Live bacteria were cultured and DNA was detected by PCR. Cellular immunity was tested by ELISPOT. RESULTS: After re-infections, culture positivity and persistence of DNA in lungs and blood were shorter. Detection of DNA at late time points indicated persistent infection in a few mice. Histamine was produced after primary and re-infections, and the level correlated with the number of viable bacteria in lung. IFN-gamma, IL-6 levels, IgG2/IgG1 ratio, IgA titres, and level of chlamydial heat-shock protein antibodies were higher after re-infections. IgM antibodies were demonstrated even after re-infections. High number of IFN-gamma-producing splenocytes was observed after the third inoculation. CONCLUSION: These results promote an understanding of the patho- and immune mechanisms after C. pneumoniae re-infections.
Authors: Tímea Mosolygó; József Korcsik; Emese Petra Balogh; Ildikó Faludi; Dezső P Virók; Valéria Endrész; Katalin Burián Journal: Inflamm Res Date: 2013-02-06 Impact factor: 4.575
Authors: Dezső P Virok; Tímea Raffai; Dávid Kókai; Dóra Paróczai; Anita Bogdanov; Gábor Veres; László Vécsei; Szilárd Poliska; László Tiszlavicz; Ferenc Somogyvári; Valéria Endrész; Katalin Burián Journal: Front Cell Infect Microbiol Date: 2019-06-12 Impact factor: 5.293
Authors: Ildikó Lantos; Valéria Endrész; Dezső Péter Virok; Andrea Szabó; Xinjie Lu; Tímea Mosolygó; Katalin Burián Journal: Biomed Res Int Date: 2018-03-25 Impact factor: 3.411