Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Design of a fluorescence polarization assay platform for the study of human Hsp70.

Literature DB >> 18515098

Design of a fluorescence polarization assay platform for the study of human Hsp70.

Yanlong Kang¹, Tony Taldone, Cristina C Clement, Sheara W Fewell, Julia Aguirre, Jeffrey L Brodsky, Gabriela Chiosis.

Abstract

The 70kDa heat shock proteins (Hsp70) are molecular chaperones that assist in folding of newly synthesized polypeptides, refolding or denaturation of misfolded proteins, and translocation of proteins across biological membranes. In addition, Hsp70 play regulatory roles in signal transduction, cell cycle, and apoptosis. Here, we present a novel assay platform based on fluorescence polarization that is suitable for investigating the yet elusive molecular mechanics of human Hsp70 allosteric regulation.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2008 PMID： 18515098 PMCID： PMC2538440 DOI： 10.1016/j.bmcl.2008.05.046

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

14 in total

Review 1. Molecular chaperones in the cytosol: from nascent chain to folded protein.

Authors: F Ulrich Hartl; Manajit Hayer-Hartl
Journal: Science Date: 2002-03-08 Impact factor: 47.728

2. Regulation of Hsp70 function by a eukaryotic DnaJ homolog.

Authors: D M Cyr; X Lu; M G Douglas
Journal: J Biol Chem Date: 1992-10-15 Impact factor: 5.157

3. cis-Effect of DnaJ on DnaK in ternary complexes with chimeric DnaK/DnaJ-binding peptides.

Authors: Wanjiang Han; Philipp Christen
Journal: FEBS Lett Date: 2004-04-09 Impact factor: 4.124

Review 4. Pathways of chaperone-mediated protein folding in the cytosol.

Authors: Jason C Young; Vishwas R Agashe; Katja Siegers; F Ulrich Hartl
Journal: Nat Rev Mol Cell Biol Date: 2004-10 Impact factor: 94.444

5. Functional specificity among Hsp70 molecular chaperones.

Authors: P James; C Pfund; E A Craig
Journal: Science Date: 1997-01-17 Impact factor: 47.728

6. The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain.

Authors: A Srinivasan; A J McClellan; J Vartikar; I Marks; P Cantalupo; Y Li; P Whyte; K Rundell; J L Brodsky; J M Pipas
Journal: Mol Cell Biol Date: 1997-08 Impact factor: 4.272

Review 7. Targeting chaperones in transformed systems--a focus on Hsp90 and cancer.

Authors: Gabriela Chiosis
Journal: Expert Opin Ther Targets Date: 2006-02 Impact factor: 6.902

Review 8. Geldanamycin as a potential anti-cancer agent: its molecular target and biochemical activity.

Authors: L Neckers; T W Schulte; E Mimnaugh
Journal: Invest New Drugs Date: 1999 Impact factor: 3.850

9. Synthesis of novel fluorescent probes for the molecular chaperone Hsp90.

Authors: Laura Llauger-Bufi; Sara J Felts; Henri Huezo; Neal Rosen; Gabriela Chiosis
Journal: Bioorg Med Chem Lett Date: 2003-11-17 Impact factor: 2.823

Review 10. Heat-shock protein 90 inhibitors as novel cancer chemotherapeutics - an update.

Authors: Len Neckers; Katharine Neckers
Journal: Expert Opin Emerg Drugs Date: 2005-02 Impact factor: 4.191

14 in total

1. High-throughput screen for Escherichia coli heat shock protein 70 (Hsp70/DnaK): ATPase assay in low volume by exploiting energy transfer.

Authors: Yoshinari Miyata; Lyra Chang; Anthony Bainor; Thomas J McQuade; Christopher P Walczak; Yaru Zhang; Martha J Larsen; Paul Kirchhoff; Jason E Gestwicki
Journal: J Biomol Screen Date: 2010-10-06

2. A cell-based screen for inhibitors of protein folding and degradation.

Authors: Frank Boschelli; Jennifer M Golas; Roseann Petersen; Vincent Lau; Lei Chen; Diane Tkach; Qiang Zhao; Dave S Fruhling; Hao Liu; Chaneun Nam; Kim T Arndt
Journal: Cell Stress Chaperones Date: 2010-08-19 Impact factor: 3.667

3. The yeast Hsp110, Sse1p, exhibits high-affinity peptide binding.

Authors: Jennifer L Goeckeler; Anthony P Petruso; Julia Aguirre; Cristina C Clement; Gabriela Chiosis; Jeffrey L Brodsky
Journal: FEBS Lett Date: 2008-06-06 Impact factor: 4.124

Review 4. Molecular chaperones and regulation of tau quality control: strategies for drug discovery in tauopathies.

Authors: Yoshinari Miyata; John Koren; Janine Kiray; Chad A Dickey; Jason E Gestwicki
Journal: Future Med Chem Date: 2011-09 Impact factor: 3.808

5. A high throughput substrate binding assay reveals hexachlorophene as an inhibitor of the ER-resident HSP70 chaperone GRP78.

Authors: Andrew J Ambrose; Christopher J Zerio; Jared Sivinski; Cody J Schmidlin; Taoda Shi; Alison B Ross; Kimberly J Widrick; Steven M Johnson; Donna D Zhang; Eli Chapman
Journal: Bioorg Med Chem Lett Date: 2019-05-20 Impact factor: 2.823

6. Identification of an allosteric small-molecule inhibitor selective for the inducible form of heat shock protein 70.

Authors: Matthew K Howe; Khaldon Bodoor; David A Carlson; Philip F Hughes; Yazan Alwarawrah; David R Loiselle; Alex M Jaeger; David B Darr; Jamie L Jordan; Lucas M Hunter; Eileen T Molzberger; Theodore A Gobillot; Dennis J Thiele; Jeffrey L Brodsky; Neil L Spector; Timothy A J Haystead
Journal: Chem Biol Date: 2014-12-11

7. Mutations in the Yeast Hsp70, Ssa1, at P417 Alter ATP Cycling, Interdomain Coupling, and Specific Chaperone Functions.

Authors: Patrick G Needham; Hardik J Patel; Gabriela Chiosis; Patrick H Thibodeau; Jeffrey L Brodsky
Journal: J Mol Biol Date: 2015-04-23 Impact factor: 5.469

8. Recent Advances in the Pathobiology of Hodgkin's Lymphoma: Potential Impact on Diagnostic, Predictive, and Therapeutic Strategies.

Authors: Diponkar Banerjee
Journal: Adv Hematol Date: 2011-01-18

9. Development of fluorescence polarization assays for the molecular chaperone Hsp70 family members: Hsp72 and DnaK.

Authors: Laura Ricci; Kevin P Williams
Journal: Curr Chem Genomics Date: 2008-12-30

10. Identification of Hsp70 modulators through modeling of the substrate binding domain.

Authors: Conor M Haney; Corinne Schneider; Barbara Beck; Jeffrey L Brodsky; Alexander Dömling
Journal: Bioorg Med Chem Lett Date: 2009-04-20 Impact factor: 2.823