Literature DB >> 18514178

Increased organophosphate scavenging in a butyrylcholinesterase mutant.

Brian C Geyer1, Ryan R Woods, Tsafrir S Mor.   

Abstract

Nicotiana benthamiana plant lines expressing a reengineered human butyrylcholinesterase (BChE) with enhanced cocaine hydrolase activity were created. Subsequent purification and biochemical analysis revealed that compared to wild-type butyrylcholinesterase, the cocaine hydrolase displayed increased affinity to the organophosphate (OP) pesticides paraoxon (6.8 4x 10(-10)M vs. 1.11 x 10(-8)M) and malaoxon (9.81 x 10(-8)M vs. 5.99 x 10(-7)M). Furthermore, the cocaine hydrolase retained identical anticholinesterase binding profiles for all other compounds tested. Thus we have demonstrated a potential large-scale production platform for a multivalent antidote for cocaine and anticholinesterase poisoning.

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Year:  2008        PMID： 18514178     DOI： 10.1016/j.cbi.2008.04.012

Source DB:  PubMed          Journal:  Chem Biol Interact        ISSN： 0009-2797            Impact factor:   5.192


  9 in total

1.  Aerosolized recombinant human butyrylcholinesterase delivered by a nebulizer provides long term protection against inhaled paraoxon in macaques.

Authors:  Yvonne Rosenberg; James Fink; Ronan MacLoughlin; Tara Ooms-Konecny; Dennis Sullivan; William Gerk; Lingjun Mao; Xiaoming Jiang; Jonathan Lees; Lori Urban; Narayanan Rajendran
Journal:  Chem Biol Interact       Date:  2019-06-12       Impact factor: 5.192

2.  Enzymatic detoxification of organophosphorus pesticides and related toxicants.

Authors:  Karla Alejo-González; Erik Hanson-Viana; Rafael Vazquez-Duhalt
Journal:  J Pestic Sci       Date:  2018-02-28       Impact factor: 1.519

Review 3.  Acetylcholinesterase inhibition resulting from exposure to inhaled OP can be prevented by pretreatment with BChE in both macaques and minipigs.

Authors:  Yvonne Rosenberg; Ashima Saxena
Journal:  Neuropharmacology       Date:  2020-05-19       Impact factor: 5.250

4.  A plant-derived cocaine hydrolase prevents cocaine overdose lethality and attenuates cocaine-induced drug seeking behavior.

Authors:  Katherine E Larrimore; Latha Kannan; R Player Kendle; Tameem Jamal; Matthew Barcus; Kathryn Stefanko; Jacquelyn Kilbourne; Stephen Brimijoin; Chang-Guo Zhan; Janet Neisewander; Tsafrir S Mor
Journal:  Prog Neuropsychopharmacol Biol Psychiatry       Date:  2020-05-06       Impact factor: 5.067

5.  Plants as a source of butyrylcholinesterase variants designed for enhanced cocaine hydrolase activity.

Authors:  Katherine E Larrimore; Matthew Barcus; Latha Kannan; Yang Gao; Chang-Guo Zhan; Stephen Brimijoin; Tsafrir Mor
Journal:  Chem Biol Interact       Date:  2012-09-20       Impact factor: 5.192

6.  Early appearance and possible functions of non-neuromuscular cholinesterase activities.

Authors:  Carla Falugi; Maria G Aluigi
Journal:  Front Mol Neurosci       Date:  2012-04-20       Impact factor: 5.639

7.  Plant-expressed cocaine hydrolase variants of butyrylcholinesterase exhibit altered allosteric effects of cholinesterase activity and increased inhibitor sensitivity.

Authors:  Katherine E Larrimore; I Can Kazan; Latha Kannan; R Player Kendle; Tameem Jamal; Matthew Barcus; Ashini Bolia; Stephen Brimijoin; Chang-Guo Zhan; S Banu Ozkan; Tsafrir S Mor
Journal:  Sci Rep       Date:  2017-09-05       Impact factor: 4.379

8.  Reversal of succinylcholine induced apnea with an organophosphate scavenging recombinant butyrylcholinesterase.

Authors:  Brian C Geyer; Katherine E Larrimore; Jacquelyn Kilbourne; Latha Kannan; Tsafrir S Mor
Journal:  PLoS One       Date:  2013-03-11       Impact factor: 3.240

9.  Plant expression of cocaine hydrolase-Fc fusion protein for treatment of cocaine abuse.

Authors:  Guojun Wang; Ting Zhang; Haifeng Huang; Shurong Hou; Xiabin Chen; Fang Zheng; Chang-Guo Zhan
Journal:  BMC Biotechnol       Date:  2016-10-19       Impact factor: 2.563
  9 in total

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