Literature DB >> 1851216

Primary culture of neonatal rat olfactory neurons.

G V Ronnett1, L D Hester, S H Snyder.   

Abstract

We have prepared primary cultures of purified neonatal rat olfactory neurons. Dissociated olfactory epithelial cells are maintained in modified Eagle's medium with D-valine, cytosine arabinoside, and NGF. NGF is required for neuronal survival. Immunohistochemical staining is positive for the neuronal markers vimentin, olfactory marker protein, and neuron-specific enolase, but negative for the glial markers, glial fibrillary acidic protein, and S-100 protein. Physiologic concentrations of odorants stimulate cAMP accumulation in the cells. Because of their morphology, biochemical composition, and responsiveness to odorants, these cells should enhance olfactory investigations.

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Year:  1991        PMID： 1851216      PMCID： PMC6575313     

Source DB:  PubMed          Journal:  J Neurosci        ISSN： 0270-6474            Impact factor:   6.167


  24 in total

Review 1.  Reconstructing smell.

Authors:  R D Barber; G V Ronnett
Journal:  Mol Neurobiol       Date:  2000-06       Impact factor: 5.590

2.  Phosphoinositide and Erk signaling pathways mediate activity-driven rodent olfactory sensory neuronal survival and stress mitigation.

Authors:  So Yeun Kim; Alex Mammen; Seung-Jun Yoo; Bongki Cho; Eun-Kyoung Kim; Jong-In Park; Cheil Moon; Gabriele V Ronnett
Journal:  J Neurochem       Date:  2015-06-08       Impact factor: 5.372

3.  In vitro generation of adult rat olfactory sensory neurons and regulation of maturation by coculture with CNS tissues.

Authors:  R J Grill; S K Pixley
Journal:  J Neurosci       Date:  1997-05-01       Impact factor: 6.167

4.  Isolation and in vitro differentiation of conditionally immortalized murine olfactory receptor neurons.

Authors:  R D Barber; D E Jaworsky; K W Yau; G V Ronnett
Journal:  J Neurosci       Date:  2000-05-15       Impact factor: 6.167

5.  Odorants induce the phosphorylation of the cAMP response element binding protein in olfactory receptor neurons.

Authors:  C Moon; Y K Sung; R Reddy; G V Ronnett
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

6.  ICA 512, an autoantigen of type I diabetes, is an intrinsic membrane protein of neurosecretory granules.

Authors:  M Solimena; R Dirkx; J M Hermel; S Pleasic-Williams; J A Shapiro; L Caron; D U Rabin
Journal:  EMBO J       Date:  1996-05-01       Impact factor: 11.598

7.  Odorant receptors at the growth cone are coupled to localized cAMP and Ca2+ increases.

Authors:  Micol Maritan; Giovanni Monaco; Ilaria Zamparo; Manuela Zaccolo; Tullio Pozzan; Claudia Lodovichi
Journal:  Proc Natl Acad Sci U S A       Date:  2009-02-13       Impact factor: 11.205

8.  Maintaining epitheliopoietic potency when culturing olfactory progenitors.

Authors:  Woochan Jang; James Lambropoulos; Jin Kyung Woo; Carolyn E Peluso; James E Schwob
Journal:  Exp Neurol       Date:  2008-07-22       Impact factor: 5.330

9.  Atrial natriuretic peptide type C induces a cell-cycle switch from proliferation to differentiation in brain-derived neurotrophic factor- or nerve growth factor-primed olfactory receptor neurons.

Authors:  P Jeanette Simpson; Ian Miller; Cheil Moon; Andrea L Hanlon; Daniel J Liebl; Gabriele V Ronnett
Journal:  J Neurosci       Date:  2002-07-01       Impact factor: 6.167

10.  Mechanism of olfactory masking in the sensory cilia.

Authors:  Hiroko Takeuchi; Hirohiko Ishida; Satoshi Hikichi; Takashi Kurahashi
Journal:  J Gen Physiol       Date:  2009-05-11       Impact factor: 4.086
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