BACKGROUND: Multiple lines of investigation have implicated hydrogen peroxide (H(2)O(2)) as an important endogenous mediator of cell proliferation in the vessel wall. Heterogeneous nuclear ribonucleoprotein C (hnRNP-C), a nuclear pre-mRNA binding protein that plays roles in vertebrate cell proliferation and differentiation, has been identified as a component of a vascular cell signaling pathway activated by low physiologic levels of H(2)O(2). The expression of hnRNP-C in human arteries has not previously been assessed. METHODS: Segments of human proximal internal carotid arteries were evaluated for the expression of hnRNP-C by immunohistochemistry. RESULTS: In normal proximal internal carotid arteries, hnRNP-C is expressed predominantly by the endothelium, with significantly lower expression by medial smooth muscle. In preatherosclerotic intimal hyperplasia, hnRNP-C is up-regulated in the artery wall, due to the robust expression by the intimal smooth muscle cells, without up-regulation in the medial smooth muscle cells. In arteries with atherosclerotic lesions, there is strong expression of hnRNP-C not only by intimal cells but also by medial smooth muscle cells. CONCLUSIONS: The H(2)O(2) responsive pre-mRNA binding protein hnRNP-C is up-regulated in atherosclerosis and in preatherosclerotic intimal hyperplasia in humans, supporting the hypothesis that H(2)O(2) is a regulator of vascular cell proliferation in these conditions. These data also suggest that hnRNP-C may be useful as a marker of vascular cell activation.
BACKGROUND: Multiple lines of investigation have implicated hydrogen peroxide (H(2)O(2)) as an important endogenous mediator of cell proliferation in the vessel wall. Heterogeneous nuclear ribonucleoprotein C (hnRNP-C), a nuclear pre-mRNA binding protein that plays roles in vertebrate cell proliferation and differentiation, has been identified as a component of a vascular cell signaling pathway activated by low physiologic levels of H(2)O(2). The expression of hnRNP-C in human arteries has not previously been assessed. METHODS: Segments of human proximal internal carotid arteries were evaluated for the expression of hnRNP-C by immunohistochemistry. RESULTS: In normal proximal internal carotid arteries, hnRNP-C is expressed predominantly by the endothelium, with significantly lower expression by medial smooth muscle. In preatherosclerotic intimal hyperplasia, hnRNP-C is up-regulated in the artery wall, due to the robust expression by the intimal smooth muscle cells, without up-regulation in the medial smooth muscle cells. In arteries with atherosclerotic lesions, there is strong expression of hnRNP-C not only by intimal cells but also by medial smooth muscle cells. CONCLUSIONS: The H(2)O(2) responsive pre-mRNA binding protein hnRNP-C is up-regulated in atherosclerosis and in preatherosclerotic intimal hyperplasia in humans, supporting the hypothesis that H(2)O(2) is a regulator of vascular cell proliferation in these conditions. These data also suggest that hnRNP-C may be useful as a marker of vascular cell activation.
Authors: Dan Sorescu; Daiana Weiss; Bernard Lassègue; Roza E Clempus; Katalin Szöcs; George P Sorescu; Liisa Valppu; Mark T Quinn; J David Lambeth; J David Vega; W Robert Taylor; Kathy K Griendling Journal: Circulation Date: 2002-03-26 Impact factor: 29.690
Authors: Katalin Szöcs; Bernard Lassègue; Dan Sorescu; Lula L Hilenski; Liisa Valppu; Tracey L Couse; Josiah N Wilcox; Mark T Quinn; J David Lambeth; Kathy K Griendling Journal: Arterioscler Thromb Vasc Biol Date: 2002-01 Impact factor: 8.311
Authors: Stephanie M Cizek; Shahinaz Bedri; Paul Talusan; Nilsa Silva; Hang Lee; James R Stone Journal: Cardiovasc Pathol Date: 2007-07-24 Impact factor: 2.185
Authors: Mikhail P Panchenko; Zakir Siddiquee; David M Dombkowski; Yuriy O Alekseyev; Marc E Lenburg; Jennifer D Walker; Thomas E Macgillivray; Frederic I Preffer; James R Stone Journal: Am J Pathol Date: 2010-08-09 Impact factor: 4.307