BACKGROUND: Circulating cell-free (ccf) DNA is measurable in healthy individuals and in higher concentration in patients with benign and malignant breast disease (BD). PATIENTS AND METHODS: In paired plasma and serum samples ccf DNA was extracted and quantified by real-time quantitative PCR for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. RESULTS: The concentration of ccf DNA in serum was higher in patients with benign and malignant BD (p = 0.023/p = 0.001) compared to healthy controls, whereas ccf DNA in plasma was higher in patients with malignant BD compared to patients with benign BD or healthy controls (p = 0.012/0.007). The ccf DNA correlated significantly between plasma and serum samples in patients with benign (p = 0.01; R: 0.677) as well as malignant BD (p = 0.01; R:0.713). CONCLUSION: The positive correlation between ccf DNA in plasma and serum in patients with benign as well as malignant BD, might have a diagnostic value for discriminating between malignant and benign BD.
BACKGROUND: Circulating cell-free (ccf) DNA is measurable in healthy individuals and in higher concentration in patients with benign and malignant breast disease (BD). PATIENTS AND METHODS: In paired plasma and serum samples ccf DNA was extracted and quantified by real-time quantitative PCR for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. RESULTS: The concentration of ccf DNA in serum was higher in patients with benign and malignant BD (p = 0.023/p = 0.001) compared to healthy controls, whereas ccf DNA in plasma was higher in patients with malignant BD compared to patients with benign BD or healthy controls (p = 0.012/0.007). The ccf DNA correlated significantly between plasma and serum samples in patients with benign (p = 0.01; R: 0.677) as well as malignant BD (p = 0.01; R:0.713). CONCLUSION: The positive correlation between ccf DNA in plasma and serum in patients with benign as well as malignant BD, might have a diagnostic value for discriminating between malignant and benign BD.
Authors: Jacqueline A Shaw; Karen Page; Kevin Blighe; Natasha Hava; David Guttery; Becky Ward; James Brown; Chetana Ruangpratheep; Justin Stebbing; Rachel Payne; Carlo Palmieri; Suzy Cleator; Rosemary A Walker; R Charles Coombes Journal: Genome Res Date: 2011-10-11 Impact factor: 9.043
Authors: Susan R Sturgeon; Raji Balasubramanian; Catherine Schairer; Hyman B Muss; Regina G Ziegler; Kathleen F Arcaro Journal: Epigenetics Date: 2012-09-17 Impact factor: 4.528
Authors: Corina Kohler; Ramin Radpour; Zeinab Barekati; Reza Asadollahi; Johannes Bitzer; Edward Wight; Nicole Bürki; Claude Diesch; Wolfgang Holzgreve; Xiao Yan Zhong Journal: Mol Cancer Date: 2009-11-17 Impact factor: 27.401