BACKGROUND: Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations. OBJECTIVES: To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations. STUDY DESIGN: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction. RESULTS: B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products. CONCLUSION: We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
BACKGROUND:Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations. OBJECTIVES: To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations. STUDY DESIGN: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction. RESULTS:B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products. CONCLUSION: We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
Authors: Marcus Vinícius Guimarães Lacerda; Victor Hugo Aquino; Mario Luis Garcia de Figueiredo; Evan P Williams; Colleen B Jonsson; Mohd Jaseem Khan; Márcio Roberto Teixeira Nunes; Clayton Pereira Silva de Lima; Luiz Tadeu Moraes Figueiredo; Mônica Regina Farias Costa; Maria Paula Gomes Mourão Journal: Arch Virol Date: 2022-07-16 Impact factor: 2.685
Authors: Judith M Hübschen; Zefira Mihneva; Andreas F Mentis; François Schneider; Yair Aboudy; Zehava Grossman; Hagit Rudich; Kalia Kasymbekova; Inna Sarv; Jasminka Nedeljkovic; Marc C Tahita; Zekiba Tarnagda; Jean-Bosco Ouedraogo; A G Gerasimova; T N Moskaleva; Nina T Tikhonova; Nazibrola Chitadze; J C Forbi; Adedayo O Faneye; Jesse A Otegbayo; Emilie Charpentier; Claude P Muller Journal: J Clin Microbiol Date: 2009-09-09 Impact factor: 5.948
Authors: Bianca E Gama; Vanessa E Emmel; Michelle Oliveira-Silva; Luciana M Gutiyama; Leonardo Arcuri; Marta Colares; Rita de Cássia Tavares; Luis F Bouzas; Eliana Abdelhay; Rocio Hassan Journal: Transplant Direct Date: 2017-10-02
Authors: Nguyen L Toan; Bui T Sy; Le H Song; Hoang V Luong; Nguyen T Binh; Vu Q Binh; Reinhard Kandolf; Thirumalaisamy P Velavan; Peter G Kremsner; C-Thomas Bock Journal: BMC Infect Dis Date: 2013-08-15 Impact factor: 3.090
Authors: M Toppinen; M F Perdomo; J U Palo; P Simmonds; S J Lycett; M Söderlund-Venermo; A Sajantila; K Hedman Journal: Sci Rep Date: 2015-11-27 Impact factor: 4.379