Literature DB >> 18500773

Transient expression of chicken alpha interferon gene in lettuce.

Li Song1, De-gang Zhao, Yong-jun Wu, Yi Li.   

Abstract

We investigated the possibility of producing chicken alpha interferon (ChIFN-alpha) in transgenic plants. The cDNA encoding ChIFN-alpha was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-alpha gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF). The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

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Year:  2008        PMID： 18500773      PMCID： PMC2367372          DOI： 10.1631/jzus.B0710596

Source DB:  PubMed          Journal:  J Zhejiang Univ Sci B        ISSN： 1673-1581            Impact factor:   3.066


  29 in total

1.  Engineering zucchini yellow mosaic potyvirus as a non-pathogenic vector for expression of heterologous proteins in cucurbits.

Authors:  T Arazi; S G Slutsky; Y M Shiboleth; Y Wang; M Rubinstein; S Barak; J Yang; A Gal-On
Journal:  J Biotechnol       Date:  2001-04-27       Impact factor: 3.307

2.  The in vivo and in vitro effects of chicken interferon alpha on infectious bursal disease virus and Newcastle disease virus infection.

Authors:  C W Mo; Y C Cao; B L Lim
Journal:  Avian Dis       Date:  2001 Apr-Jun       Impact factor: 1.577

3.  [Cloning, prokaryotic expression of chicken interferon-alpha gene and study on antiviral effect of recombinant chicken interferon-alpha].

Authors:  Qin Wei; Gui-Qing Peng; Mei-Lin Jin; Yu-Dong Zhu; Hong-Bo Zhou; Hong-Yan Guo; Huan-Chun Chen
Journal:  Sheng Wu Gong Cheng Xue Bao       Date:  2006-09

4.  Cellular responses in chickens treated with IFN-alpha orally or inoculated with recombinant Marek's disease virus expressing IFN-alpha.

Authors:  K W Jarosinski; W Jia; M J Sekellick; P I Marcus; K A Schat
Journal:  J Interferon Cytokine Res       Date:  2001-05       Impact factor: 2.607

5.  Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks.

Authors:  Mudasser Habib; Iftikhar Hussain; Hamid Irshad; Zong-zhao Yang; Jiang-bing Shuai; Ning Chen
Journal:  J Zhejiang Univ Sci B       Date:  2006-08       Impact factor: 3.066

6.  A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens.

Authors:  Qiyun Zhu; Huanliang Yang; Weiye Chen; Wenyan Cao; Gongxun Zhong; Peirong Jiao; Guohua Deng; Kangzhen Yu; Chinglai Yang; Zhigao Bu; Yoshihiro Kawaoka; Hualan Chen
Journal:  J Virol       Date:  2007-10-17       Impact factor: 5.103

7.  Establishment and maintenance of the innate antiviral response to West Nile Virus involves both RIG-I and MDA5 signaling through IPS-1.

Authors:  Brenda L Fredericksen; Brian C Keller; Jamie Fornek; Michael G Katze; Michael Gale
Journal:  J Virol       Date:  2007-10-31       Impact factor: 5.103

8.  Multiple anti-interferon actions of the influenza A virus NS1 protein.

Authors:  Georg Kochs; Adolfo García-Sastre; Luis Martínez-Sobrido
Journal:  J Virol       Date:  2007-04-18       Impact factor: 5.103

9.  Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS).

Authors:  Zahid Iqbal Rajput; Chen-wen Xiao; Song-hua Hu; Abdullah G Arijo; Noor Mohammad Soomro
Journal:  J Zhejiang Univ Sci B       Date:  2007-05       Impact factor: 3.066

10.  Bird migration routes and risk for pathogen dispersion into western Mediterranean wetlands.

Authors:  Elsa Jourdain; Michel Gauthier-Clerc; Dominique J Bicout; Philippe Sabatier
Journal:  Emerg Infect Dis       Date:  2007-03       Impact factor: 6.883
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  4 in total

1.  High-level expression of human interferon alpha-2b in transgenic carrot (Daucus carota L.) plants.

Authors:  Yu Luchakivskaya; O Kishchenko; I Gerasymenko; Z Olevinskaya; Yu Simonenko; M Spivak; M Kuchuk
Journal:  Plant Cell Rep       Date:  2010-11-03       Impact factor: 4.570

2.  Expression of biologically active human interferon alpha 2 in Aloe vera.

Authors:  William Lowther; Kevin Lorick; Susan D Lawrence; Wen-Shuz Yeow
Journal:  Transgenic Res       Date:  2012-04-24       Impact factor: 2.788

Review 3.  Recombinant cytokines from plants.

Authors:  Agnieszka Sirko; Tomas Vaněk; Anna Góra-Sochacka; Patrycja Redkiewicz
Journal:  Int J Mol Sci       Date:  2011-06-03       Impact factor: 5.923

Review 4.  Insight into alternative approaches for control of avian influenza in poultry, with emphasis on highly pathogenic H5N1.

Authors:  E M Abdelwhab; Hafez M Hafez
Journal:  Viruses       Date:  2012-11-19       Impact factor: 5.048
  4 in total

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