Literature DB >> 1850044

Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity.

T M Dmitrieva1, K B Norkina, V I Agol.   

Abstract

RNA template- and primer-dependent preparations of RNA polymerase were purified from encephalomyocarditis virus-infected Krebs-2 cells, using a three-step chromatographic procedure. The RNA duplex-unwinding activity of these preparations was investigated by two assays, using a partially double-stranded RNA template (encephalomyocarditis virus RNA annealed with a long segment of antisense transcript). Less purified preparations of the polymerase appeared to be able to efficiently displace, in an ATP-dependent and RNA elongation-dependent reaction, the antisense segment from the template. However, upon a more extensive purification, the unwinding activity of the RNA polymerase preparations was lost.

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Year:  1991        PMID： 1850044      PMCID： PMC240635     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  14 in total

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Authors:  E Wimmer; R J Kuhn; S Pincus; C F Yang; H Toyoda; M J Nicklin; N Takeda
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3.  Synthesis of plus- and minus-strand RNA from poliovirion RNA template in vitro.

Authors:  T D Hey; O C Richards; E Ehrenfeld
Journal:  J Virol       Date:  1986-06       Impact factor: 5.103

4.  On the mechanism of single-stranded RNA synthesis by encephalomyocarditis virus replication complexes: preferential inhibition by adenylyl (beta, gamma-methylene)-diphosphonate.

Authors:  T M Dmitrieva; T P Eremeeva; G I Alatortseva; V I Agol
Journal:  FEBS Lett       Date:  1980-06-16       Impact factor: 4.124

5.  Dependence of the activity of the poliovirus replicase on the host cell protein.

Authors:  A Dasgupta; P Zabel; D Baltimore
Journal:  Cell       Date:  1980-02       Impact factor: 41.582

6.  Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.

Authors:  J B Flanegan; T A Van Dyke
Journal:  J Virol       Date:  1979-10       Impact factor: 5.103

7.  Primer-dependent synthesis of covalently linked dimeric RNA molecules by poliovirus replicase.

Authors:  J M Lubinski; L J Ransone; A Dasgupta
Journal:  J Virol       Date:  1987-10       Impact factor: 5.103

8.  Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro.

Authors:  D C Young; D M Tuschall; J B Flanegan
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

9.  Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.

Authors:  T A Van Dyke; J B Flanegan
Journal:  J Virol       Date:  1980-09       Impact factor: 5.103

10.  Genome-length copies of poliovirion RNA are synthesized in vitro by the poliovirus RNA-dependent RNA polymerase.

Authors:  T A Van Dyke; R J Rickles; J B Flanegan
Journal:  J Biol Chem       Date:  1982-04-25       Impact factor: 5.157
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  5 in total

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2.  RNA duplex unwinding activity of poliovirus RNA-dependent RNA polymerase 3Dpol.

Authors:  M W Cho; O C Richards; T M Dmitrieva; V Agol; E Ehrenfeld
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

Review 3.  Picornavirus nonstructural proteins: emerging roles in virus replication and inhibition of host cell functions.

Authors:  A G Porter
Journal:  J Virol       Date:  1993-12       Impact factor: 5.103

4.  An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis.

Authors:  Dorothee A Vogt; Raul Andino
Journal:  PLoS Pathog       Date:  2010-06-03       Impact factor: 6.823

Review 5.  Genetic analysis of picornaviruses.

Authors:  K Kirkegaard
Journal:  Curr Opin Genet Dev       Date:  1992-02       Impact factor: 5.578
  5 in total

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